中国组织工程研究与临床康复2011，Vol.15Issue(50)：9425-9427,3.DOI:10.3969/j.issn.1673-8225.2011.50.028
小鼠热休克转录因子1真核表达载体的构建
Construction of a eukaryotic expression vector containing mouse heat shock transcription factor 1
摘要
Abstract
BACKGROUND: Htit shook tfansoription factor 1 his hut shock rasponst tfftotOBIECTWE: To oonstniot I tukaiyotfe txprtsslon vtotor containing moust htit shock ti inscription fiotoi 1 (HSF1).kETHOOS:Amc4?tdmoustHSF1oDNAfiigmtntMisolont(llntopoDNA3.1tooonstniotituk>yo?otxpitsslonvtoloiP00NA3.1-HSF1.RESULTS AND CONCLUSION: ThtttMtrttMofragmtnfc In clcct.opho.css atte. .esl.iction enzyme <igtsion.5.5kbtagmtntind 1￡ kb fiigmtnt The result of DNAs tqutnoing showed that the secuence of the cloned HSF1 MB identical to thatStntBai* had .epoited HSF1 piotein band wifi the .dative mokoulai mass of 72000mb detected by Mtstttn blot Thesefindings indicate that the e<* aiyoiic cxp.ession vtotoi poDNA3.1-HSF1 oontahlng moust HSF1 Is successfully consl.ucted.关键词
热休克转录因子1/真核表达载体/基因重组/小鼠/组织工程分类
医药卫生引用本文复制引用
欧阳华伟,谭军,李高峰,罗成群..小鼠热休克转录因子1真核表达载体的构建[J].中国组织工程研究与临床康复,2011,15(50):9425-9427,3.基金项目
国家自然科学基金项目(30672035).项目名称:从HSF1水平探讨烧伤炎症反应中细胞内源性分子保护机制. （30672035）
