浙江医学2012，Vol.34Issue(1)：12-14,3.
西地那非对离体大鼠膀胱颈平滑肌的舒张作用及途径
Relaxation effects of sildenafil on rat isolated bladder neck smooth muscle and its mechanism
摘要
Abstract
To investigate the relaxation effects of sildenafil on rat isolated bladder neck smooth muscle and itsmechanism.MethodsTwenty four adult male Sprague Dawley rats (SD) weighted 230 ~ 280g were randomly divided into 4groups. Bladder neck smooth muscles were isolated in all groups and firstly precontracted with norepinephrine. Then the isolated smooth muscles were treated with different concentration of sildenafil (sildenafil group); sildenafil with Nw-nitro-L-argi-nine-methyl-ester (L-NAME+sildenafil group); sildenafil with 1H- [1,2,4]oxadiazolo [4,3-a]quiNOxalin-1-one (ODQ+sildenafil group); or sildenafil with zinc protoporphyrin (ZnPP+sildenafil group).ResultsSildenafil induced concentration-dependent re-laxation on rat isolated bladder neck smooth muscle which was precontracted with norepinephrine, the maximum rate of relaxation (Emax) was (91.753 ±2.457)% with a IC50 of (6.150 ± 0.784) x 10-7mol/L Compared with the sildenafil group, the Emaxof the other three groups decreased markedly, and the IC50 increased significantly (P<0.05).The Emax of L-NAME+ sildenafil group and ZnPP+ sildenafil group was higher than that of ODQ+ sildenafil group significantly, and the IC50 was lower (P<0.05).Sildenafil can induce concentration-dependent relaxation of rat bladder neck smooth muscle. This effect can be blocked by phosphodiesterase type 5 (PDE5) inhibitors L-NAME, ODQ and ZnPP, indicating the involvement of NO-cGMP pathway or CO-cGMP pathway.关键词
大鼠膀胱颈平滑肌/西地那非/NO/CO/cGMPKey words
Rat bladder neck smooth muscle/Sildenafil/NO CO cGMP引用本文复制引用
叶雪挺,陈洪德,翁志梁,李澄棣..西地那非对离体大鼠膀胱颈平滑肌的舒张作用及途径[J].浙江医学,2012,34(1):12-14,3.基金项目
温州市科技局资助项目(Y20110182) （Y20110182）
