中国组织工程研究与临床康复2011，Vol.15Issue(50)：9344-9347,4.DOI:10.3969/j.issn.1673-8225.2011.50.009
人胸腺组织原代培养细胞与人类免疫缺陷病毒间的相互作用
Interaction between primary cultured human thymocytes and human immunodeficiency virus
摘要
Abstract
BACKGROUND: Human immunodeficiency virvs binds to CD4 receptors in human thymocytes, and thus causes activitydecreasing in CF=D4 celts.OBJECTIVE: To explore the relationship between human embryo thymocytes and human immunodeficiency virus.METH ODS: Primary cultured human embryo thymocytes were divided into experiment group and control group. Experimentalthymocytes were mixed cultured with human immunodeficiency virus 1 III B. Controlthymocytes were cultured without humanimmunodeficiency virus.RESULTSANDCONCLUSION: According to trans miss io n electron microscopy, mass we human immunodeficiency virus particlesemerged in thymocytes treated with human immunodeficiency virus at the 40h hour after cutivation.According to MTT assay andimmunohistochemic al staining, the absorb anee in thymocytes treated with human immunodeficiency virus was significantly lowerthan that in the control thymocytes at the 40h hour to the 2nd day after cultivation (r=0.9733. P<0.06) The Fas-Lpositive rate ofthe experiment thymocytes treated with human immunodeficiency virus increased on cell membranes and in cytoplasm (p <0.05).These findings indicate that human immunodeficiency virus decreases the cell activity in primary cultured human embryothymocytes.关键词
人类免疫缺陷病毒/胸腺/原代培养/细胞活性/组织构建分类
医药卫生引用本文复制引用
高楠竣,陈英杰,李雪涛,孔维云,周轶平..人胸腺组织原代培养细胞与人类免疫缺陷病毒间的相互作用[J].中国组织工程研究与临床康复,2011,15(50):9344-9347,4.基金项目
云南省科委基金资助项目、云南省科技厅-昆明医学院联合专项基金资助项目(98C014Q,2007C0044R,2009CD188) （98C014Q,2007C0044R,2009CD188）
云南省高校药学重点实验室创新平台建设专项基金(6010902009-2010ZL004) （6010902009-2010ZL004）
云南省教育厅科学研究基金立项资助项目(2010Y191,07Z10199). （2010Y191,07Z10199）
