中国组织工程研究与临床康复2011，Vol.15Issue(50)：9348-9352,5.DOI:10.3969/j.issn.1673-8225.2011.50.010
人类膀胱移行上皮细胞的体外培养与鉴定
Culture and identification of human bladder transitional cells in vitro
摘要
Abstract
BACKGROUND: Methods to separate the trans itional epithelial cells include enzyme digestion method, tissue block method and spinning scraping method.OBJECTIVE: To investigate the best approach to culture and passage the human bladder transitional cells in vitro. METHODS: Human bladder transitional epithelial cells were cultured in MEM-F12 and KSFM media. Then the cell morphologicalstaining based on the presence of uothelium specific cell markers AE1 and Uroplakin III, the latter onespecifically expressed in urothelialtissue. The cells were subcultured by4-step trypsin digestion method and tissue block replantation method, respectively. RESULTS AND CONCLUSION: The cells in EME-F12 media grew well, and the cell climb ing-out and cel fusion occurred earlier than cells in KSFM media. The cells were identified as transitional epithelial cells by immunofluorescence. Passage cells subcultured by trypsin digestion began to adhere to the wall at 13-24 hours after passage. The cells stopped growing after adherence. Cellapoptosis appeared after 60-72 hours. The passage cells subcultured by tissue block replantation displayed a stable and fast growth. The cell began to climb out at 24-43 hours. Cell fusion rate achieved 30% on 10-16 days. Cells beganto degenerate at the fourth passage. These result demonstrate thatissue block cultrivation and replantation based on DMEM-F12 isan econornic and effective way to culture and subculture the human bladder transitional cells.关键词
膀胱/上皮细胞/培养基/细胞培养/组织分类
医药卫生引用本文复制引用
郝维平,姚友生,王家伟,邓毕华,吕夷松..人类膀胱移行上皮细胞的体外培养与鉴定[J].中国组织工程研究与临床康复,2011,15(50):9348-9352,5.基金项目
广东省科技基金:间质性膀胱炎的病因和特异性诊断的研究(2007B13504008) （2007B13504008）
APF、Vroplakins Occludin （）
ZO-1在间质性膀胱炎发病机制中的作用探讨(2009B030801148). （2009B030801148）
