医药导报2012，Vol.31Issue(1)：1-4,4.DOI:10.3870/yydb.2012.01.001
安络化纤丸联合γ-干扰素治疗小鼠血吸虫性肝纤维化的实验研究
Experimental Research of Anluohuaxian Pill Plus Interferon-γ on Schistosomal Liver Fibrosis in Mice
摘要
Abstract
Objective To compare the effects of anluohuaxian pill plus interferon-'Y against schistosomal liver fibrosis with anluohuaxian pill used alone. Methods Fifty Kunming mice were divided into 5 groups. Except 10 mice in the normal control, the other 40 were infected with 40 Schistosoma japonicum per mouse. Six weeks later, the model of schistosomal liver fibrosis was successfully induced, and the model mice were randomly divided into 4 groups evenly. (T) The model control mice were treated by intragastric administration of 0.75 mL normal saline at each time point;①In the anluohuaxian pill group, mice were I. G. Treated with anluohuaxian pill mixed with normal saline at the same volume as above; In the interferon-'Y group, mice were hypodermically injected with 0. 1 mL mixture of interferon-γ ( 2×106 U ) and pyrogen-free water( 4 mL );④ In the two-drug combination group, mice were treated with half-and-half anluohuaxian pill and interferon-'Y one time per day for 8 weeks. By the end of therapy, the liver tissues were taken for pathological examination and expression of type I , Ⅲ collagen and TIMP-l( tissue inhibitor of metalloproteinases 1 ) was detected. Results The apparently diminished size of schistosomal egg nodules of the anluohuaxian pill group, the interferon-γ group, the two-drug group, the model control were ( 460. 406 5 ± 36. 276 8 ), ( 443. 927 6±44. 209 5 ),( 433. 430 0±64. 928 4 ),( 533. 765 8±88. 102 2 )μm, respectively. Compared with the model control, the hepatic injury was attenuated in all therapeutic groups with apparently diminished size of schistosomal egg nodules, P<0.05. Further, the expression of type I , Ⅲ collagen as well as TIMP-1 were all conspicuously reduced, especially in the two-drug combined group( P<0. 05 ). Conclusion Combination of anluohuaxian pill with interferon-'Y shows more effective on treating schistosomal liver fibrosis, which is possibly associated with inhibition of hepatic stellate cell activation and diminished expression of TIMP-1.关键词
安络化纤丸/γ-干扰素/日本血吸虫/肝纤维化/基质金属蛋白酶组织抑制因子Key words
Anluohuaxian pill/Interferon-γ/Schistosoma japonicum/Liver fibrosis/Tissue inhibitor of metalloproteinasesl分类
医药卫生引用本文复制引用
肖非,马科,黄海燕,艾国,焦云桃,宁琴,黄加权..安络化纤丸联合γ-干扰素治疗小鼠血吸虫性肝纤维化的实验研究[J].医药导报,2012,31(1):1-4,4.基金项目
湖北省血吸虫病防治项目(XF06c28) （XF06c28）
湖北省卫生厅血吸虫病防治专项(XF2008-11) （XF2008-11）
