中国药业2012，Vol.21Issue(1)：8-9,2.
高效液相色谱-质谱联用法测定大鼠血浆中五味子醇乙的浓度及其应用
Determination of Gomisin A in Rat Plasma by HPLC-MS and Its Application
张颖 1张起辉 2姜鸿 1邹桂欣 1尤献民 1毕开顺3
作者信息
- 1. 辽宁省中医药研究院,辽宁沈阳,110034
- 2. 重庆大学化学化工学院,重庆,400030
- 3. 沈阳药科大学,辽宁,沈阳,110016
- 折叠
摘要
Abstract
Objective To develop a fast and sensitive HPLC - MS method for the determination of the concentration of gomisin A in rat plasma and study the pharmacokinetics in rats. Methods Plasma was extracted by diethyl ether, and then separated on Hypersil-C18(150 mm ×4. 6 mm, 5 μm)with a mobile phase of method-water (72 : 28). Diazepam was used as the internal standard, m/ z 399.00 and m/z 284.90 were used to qualify gomisin A and diazepam, respectively. Results The linear calibration curve was obtained from 5.0 to 250 ng/mL,( r = 0. 995 7 ). The limit of quantification was 5. 0 ng/mL. The recovery for gomisin A was greater than or equal to 78.80%, the inter-day and intra - day precision (BSD) were all less than 15% (n = 6). Conclusion The method is selective, sensitive, and suitable for the preclinical pharmacokinetics of gomisin A.关键词
五味子醇乙/高效液相色谱-质谱联用法/药动学/五味子Key words
gomisin A/ HPLC - MS/ pharmacokinetics/ Fructus Schisandrae Chinensis分类
医药卫生引用本文复制引用
张颖,张起辉,姜鸿,邹桂欣,尤献民,毕开顺..高效液相色谱-质谱联用法测定大鼠血浆中五味子醇乙的浓度及其应用[J].中国药业,2012,21(1):8-9,2.
