中国组织工程研究与临床康复2011，Vol.15Issue(51)：9575-9578,4.DOI:10.3969/j.issn.1673-8225.2011.51.018
原位生长纳米羟基磷灰石晶须增强β-磷酸三钙多孔支架的生物安全性
Biological security of nano-hydroxyapatite wisker and β-tricalcium phosphate composited bone substitute
摘要
Abstract
BACKGROUND: The technique, which reinforces porous calcium phosphate scaffold by nano hydroxylapatite whisker in situ,can enhance the strength and toughness of scaffold.OBJECTIVE: To evaluate the biocompatibility of the nano-hydroxyapatite wisker and ?-tricalcium phosphate (nHAW/?-TCP) as tissue engineered vascular scaffold in vitro.METHODS: The acute systemic toxicity test: Totally 30 mice were divided into intravenous injection group, intra -abdominal injection group and control group. Mice in the three groups were injected by leaching liquor and saline respectively, then the general state of the mice was recorded at the 24, 48 and 72 hours. Test of hemolysis: Dissolution of red blood cells were observed in the mixed liquids of leaching liquor and diluted blood, and the A value was detected at optical density 545 nm then the hemolytic rare was calculated. The sensitization test: Sixteen guinea pigs were randomly divided into experimental group,negative control group and postive control group. Leaching liquor from nHAW/?-TCP, normal saline and 2% dinitrofluorobenzene were injected intradermally into each side of the backbone of each guinea pig. The erythema and edema were recorded at immediate, 24, 48, 72 hours. Test of cytotoxicity: The cells were cultured in the special L-DMEM that nHAW/?-TCP were dipped in and then general morphology of cells and cell activity were observed through microscope and CCK -8.RESULTS AND CONCLUSION: The acute systemic toxicity test: After intravenous and intra -abdominal injection of soaked of materal, there were no changes in breath, appetite, body weight of mice and no death. Test of hemolysis: The haemolytic rates of nHAW/?-TCP were less than 5% of international standard organization requirements, suggesting these materaials had no haematolysis effect. The sensitization test: No allergic reaction were found in guinea pig after endodermal injection. Test of cytotoxicity: Test of CCK -8 colormetry showed that the different densities leaching liquor had no cytotoxicity. nHAW/?-TCP does not cause acute whole body toxic reaction, haematolysis effect, allergic reaction and no cytotoxity. The materials have a good biocompatibility and meet the application requirements for tissue engineered vascular scaffold.关键词
晶须/羟基磷灰石/磷酸三钙/人工骨/生物相容性分类
医药卫生引用本文复制引用
胡红涛,许国华,刘继春,蒋玉权,唐德志,昝青峰,叶晓健..原位生长纳米羟基磷灰石晶须增强β-磷酸三钙多孔支架的生物安全性[J].中国组织工程研究与临床康复,2011,15(51):9575-9578,4.基金项目
国家自然科学基金资助项目(30872639,81071477) （30872639,81071477）
上海市科学与技术委员会基金资助项目(1052nm03200). （1052nm03200）
