中国组织工程研究与临床康复2011，Vol.15Issue(53)：9973-9976,4.DOI:10.3969/j.issn.1673-8225.2011.53.023
种属和发育期及不同实验条件对卵裂期胚胎冷冻复苏结局的影响
Effects of species, development periods and different experimental conditions on the outcomes of embryo freezing and thawing in cleavage stage
摘要
Abstract
BACKGROUND: In the studies of frozen-thawed human cleavage embryos, whether the experimental conditions and experimental animal model have the same sens ft h/ity with human to reflect the experiment quality e worth exploring. OBJECT P/E: To investigate the effect of embryo species, development periods and drffererrt frozen ccndrticns on the outcomes of embryo freezing and thawing in cleavage stage.METHODS: Human embryos in cleavage stage served as control group, KM mouse embryos were randomly divided into 2-cell-stage group. 4-cell-stage group and 8-cell-stage group. Experimentscheme: Operating environmenttemperaturewere 1S-2O "C, 24-26 "C and 37 "C. Slow programmed cryopreservation. self-made Straw leaf vitrification cryopreservation and close pulled straw vitrification ciyopreservation were used in the study. Contact durations of vitrified solution were less than 40 seconds. 40-60 seconds and 60-90 seconds. The embryo survival rates and developmental rates after cultivating for 24hours were compared among groups.RESULTS AND CONCLUSION: The survival rate of control group underthe environment temperature of 24-26 "C was hgter than that of 37 TC temperature^ < 0.05). The survival rate of control group and4-cell-stage group by serf-made Strawleaf vitrification cryopreservation was higher than that of the two groups byslow programmed cryopreservation (f < 0.05). The embryo developmental rates after cultivating for 24 hours showed no significant difference (P >005). The re was no s ign'rficant difference in embryo survival rates among groups with different contact durations of vitrified solution (p>QQ5f. These fridhgs suggest that the cleavage embryo resuscitation effect of vitrification is better than that of slow recovery process. Under the temperature of 24-26 "C, the outcomes of embryo freezing andthawing can be improved by reducing cryoprotectant dose and contact duration. Different embryo species and developmental stages may produce different outcomes under the same conditions. Compared with other stages of embryo development. 4-eel I mouse embryo t- a mores uitableexperimentalmodel for the freezing and thawing studies of human cleavage stage embryos.关键词
胚胎/玻璃化/冷冻/种属/发育期/复苏分类
医药卫生引用本文复制引用
巩晓芸,赵静,胡泊,王鹏,蔡霞..种属和发育期及不同实验条件对卵裂期胚胎冷冻复苏结局的影响[J].中国组织工程研究与临床康复,2011,15(53):9973-9976,4.基金项目
新疆医科大学第一附属医院青年专项基金(2010QN01)资助项目. （2010QN01）
