中国组织工程研究与临床康复2011，Vol.15Issue(51)：9633-9636,4.DOI:10.3969/j.issn.1673-8225.2011.51.031
胆甾醇疏水改性的羧乙基普鲁兰多糖合成及自组装性质
Synthesis of cholesterol-modified pullulan and its self-assembled behavior
摘要
Abstract
BACKGROUND: Carboxyl group is introduced to hydrophobically modify polysaccharides, which could self-aggregate to nanoparticles with some surface charges, and it is beneficial to improving their stability and performing the good properties as a drug carrier.OBJECTIVE: To synthesis cholesterol hydrophobically modified carboxyethyl pullulan (CHS -CEP), and to study their self-aggregated behavior in aqueous solution and characteristics of CHS -CEP nanoparticles.METHODS: Acrylic acid and pullulan polysaccharides could form carboxyethyl pullulan (CEP) by additive reaction. Objective material could be obtained by catalyzing CEP and CSN. The synthesized material was characterized using proton magnetic resonance (1H-NMR). The nanoparticles were prepared by dialysis to characterize the morphology, size and zeta potential by transmission electron microscopy (TEM) and dynamic laser scattering (DLS).RESULTS AND COUCLUSION: By 1H-NMR analysis, it showed that CHS-CEP was successfully synthesized and this material could self-aggregate to form nanoparticles wi th opalescence. The diameter of CHS -CEP nanoparticles was about 148.6 nm and their zeta potential was -19.9 mV. They could act as drug carrier due to many excellent properties such as a good dispersity in aqueous solution, even distribution and long-term stability.关键词
透射电镜/动态光散射分析仪/药物载体/羧乙基普鲁兰多糖/胆甾醇/纳米粒子分类
医药卫生引用本文复制引用
陶晓军,唐红波,凌凯,王艳,张其清..胆甾醇疏水改性的羧乙基普鲁兰多糖合成及自组装性质[J].中国组织工程研究与临床康复,2011,15(51):9633-9636,4.基金项目
国家重大科学研究计划项目(2006CB933300) （2006CB933300）
博士点基金(20091106120051). （20091106120051）
