中国免疫学杂志2012，Vol.28Issue(1)：45-48,53,5.DOI:10.3969/j.issn.1000-484X.2012.01.010
慢性排斥肾移植大鼠继发对自身组织蛋白vimentin的细胞和体液免疫反应
Progression of autoimmune responses against vimentin in allograft rat recipients undergoing chronic rejection
摘要
Abstract
Objective: To investigate the cellular and humoral immune responses against autoimmune tissue antigen vimentin in allograft rat recipients undergoing chronic rejection. Methods: Chronic rejection model of allograft kidney was established that the left kidney of Lewis was replaced by a F344 allograft and a contralateral nephrectomy was performed 7 days later. Proteinuria changes of recipients were observed every 2 weeks post-transplantation. The rats were sacrificed at 14th, 49th and 98th day after operation, and the renal grafts, spleens and serums were harvested. Light microscopic examination of kidney lesions was performed. The frequencies of specific IFN-γ secreting T cells in presence of vimentin were detected by ELISPOT assay, and the levels of vimentin IgG antibodies were analyzed by ELISA. Syngenic transplants Lewis rats were persisted in proteinuria measurement, and the renal grafts, spleens and serums were examined at 98th day after operation. Results:In the allograft recipients, in parallel to the proteinuria increasing, the interstitial fibrosis and tubular atrophy of kidneys with chronic allograft nephropathy ( CAN ) was developing from day 49. No proteinuria increasing and CAN were emerged in syngenic transplants at day 98. The number of vimentin-specific IFN-7 secreting T cells began to increase at day 49 ( 28. 3 ± 2.0 vs. 10. 1 ± 2. 1 ) in the allograft recipients, and significantly increased at day 98 ( 126. 0 ± 10. 4 vs. 26. 3 ±4. 1, P <0. 01 ). But in syngenic recipients lacked for the number changes. The sera ELISA analysis revealed a strong production of vimentin IgG antibodies at 14 days ( 0. 230 ±0.018 vs. 0.063 ±0. 008,P <0. 05 ) in the allograft recipients, then slowly in-creased in later period. The assays did not detect IgG antibodies to vimentin in syngenic transplants at day 98. Conclusion: Cellular and humoral autoimmune responses against vimentin occurred in rat recipients undergoing chronic rejection. The novo autoimmne after transplantation appears to be involved in CAN.关键词
自身免疫/波形蛋白/肾移植/慢性排斥/大鼠Key words
Autoimmune/vimentin/Kidney transplantation/Chronic rejection/Rat分类
医药卫生引用本文复制引用
方成,李琦,刘立,陈薇,胡汉宁,梁慧芳,王加谋,瞿永华,谢德芳..慢性排斥肾移植大鼠继发对自身组织蛋白vimentin的细胞和体液免疫反应[J].中国免疫学杂志,2012,28(1):45-48,53,5.基金项目
本文受湖北省自然科学基金(2008CDB150)资助 （2008CDB150）
