临床神经外科杂志2011，Vol.8Issue(6)：292-295,4.DOI:10.3969/j.issn.1672-7770.2011.06.004
PIAS3过表达抑制人脑胶质瘤U251细胞生长
PIAS3 overexpression inhibit the growth of human glioma U251 cells
周金方 1贡伟一 1石磊1
作者信息
- 1. 215300,江苏大学附属昆山医院神经外科
- 折叠
摘要
Abstract
Objective To investigate the function and mechanism of protein inhibitor of activated signal transducer and activator of transcription 3 ( PIAS3 ) overexpression on the growth of U251 cells. Methods The expression of PIAS3 on normal brain tissue and human glioma U251 cells was quantified by real-time fluorescence quantitative RT-PCR. U251 cells were transfected with PIAS3 overexpression vectors to improve the level of PIAS3 expression in U251 cells and then the cell proliferation was measured by MTT assay. The relationship between PIAS3 expression and proliferation-related genes PI3K and Akt was identified by Western blot. Results PIAS3 was relatively highly ex-pressied in normal brain tissue, but obviously downregulated in U251 cells ( P < 0. 01) . And our results showed transfection with PIAS3 overexpression not only remarkably induced proliferation inhibition of U251 cells,but also inhibited the PI3K activity and p-Akt expression. However,the expression of total Akt was not affected. Conclusions The low-expression of PIAS3 functions an important role on the state of proliferation in U251 cells,and upregulation the expression of PIAS3 can effectively inhibit the proliferation of U251 cells through inhibiting the activation of PI3K/Akt pathway.关键词
胶质瘤/PIAS3/增殖Key words
Gioma/ PIAS3 / proliferation分类
医药卫生引用本文复制引用
周金方,贡伟一,石磊..PIAS3过表达抑制人脑胶质瘤U251细胞生长[J].临床神经外科杂志,2011,8(6):292-295,4.
