局解手术学杂志2012，Vol.21Issue(1)：14-17,4.
实验性大鼠脑血管痉挛动物模型制备
Preparation of experimental rat model for cerebral vasospasm
摘要
Abstract
Objective To explore ihe preparation melhod for experimenlal Sprague-Dawley ral model of cerebral vasospasm afler sub-arachnoid hemorrhage (SAH). Methods The Lail artery blood was acquired and ihe arterial blood was injecled inlo cislerna magna Lwice following prone positioning in slereolaxic apparatus. India ink was infused inlo middle cerebral artery (MCA) , inlernal carolid artery (ICA) and basilar artery ( BA) Lo measure iheir diamelers. Morris waler maze LesL was applied Lo measure ihe conlenl of blood endolhelin-1 ( ET-1) and nilricoxide synlhase (NOS). Results The blood vessel of ihe brain was obviously allenualed in SAH group. The sludy and memorizing abilities were decreased according lo ihe resulls of ihe Morris waler maze lesl. The diamelers of MCA, ICA and BA were (169. 33 ± 8. 67 ) mm, (227. 33 ±14.25) mm and (226. 33 ±5. 99) mm; ihe conlenl of ET-1 and NOS in blood were (214. 36 ± 10. 49) g/L and (211. 15 ± 16.99) U/mL, slalislically different form ihose in conlrol group. Conclusion This melhod can make ihe model of vasospasm afler sub-arachnoid hemorrhage.关键词
蛛网膜下隙出血/脑血管痉挛/Morris水迷宫/动物模型/大鼠Key words
subarachnoid hemorrhage/ vasospasm/ Morris waler maze/ animal model/ ral分类
医药卫生引用本文复制引用
蔡志平,石素琴,李朝晖,崔慧先,贾建新,霍东升,罗永胜..实验性大鼠脑血管痉挛动物模型制备[J].局解手术学杂志,2012,21(1):14-17,4.基金项目
河北省自然科学基金项目(C2009001081) （C2009001081）
