重庆医学2012，Vol.41Issue(2)：153-154,157,3.DOI:10.3969/j.issn.1671-8348.2012.02.020
大鼠骨骼双染标本的制作方法
The methods of the rat skeleton double stain andpreparation*
摘要
Abstract
Objective To discuss the methods of rat skeleton double stain,in order to provide basic testing methods for making display samples for teaching or studying the normal skeleton morphological development and the skeletal deformity of embryos. Methods The 18 d fetal rat and filial rat 1 d after birth were removed skin,subcutaneous adipose tissue and viscera,and their cartilages were first dyed by the dye solution of Alcian blue,and then dyed by the dye solution of alizarin red containing potassium hydroxide, and finally dehydratsed by several levels of glycerin,made transparent and stored. Results The ossificated skeletons were dyed to red or amaranth by the alizarin red,the cartilages were dyed to blue by the Alcian blue,and other tissues were transparent. The development morphology of the skeleton could be clearly observed through stereoscope. Conclusion The dual dying method that adopts alizarin red Alcian blue could achieve the best dying effect on the skeletons and cartilages of complete fetal rats and filial rats whoes skins,subcutaneous adipose tissue and viscera had been removed,so it is a relatively direct method to study the development of skeletons and cartilages,and also a good method for making display samples for teaching.关键词
大鼠/骨骼/软骨/双染法/注意事项Key words
rat/skeleton/cartilage/dual dying method/precautions引用本文复制引用
张宏,李啸红..大鼠骨骼双染标本的制作方法[J].重庆医学,2012,41(2):153-154,157,3.基金项目
贵州省科技厅科学技术基金项目 [科合J字(2009)2215]. （2009）
