中国病理生理杂志2012，Vol.28Issue(1)：70-75,6.DOI:10.3969/j.issn.1000-4718.2012.01.013
黄芩苷对CA46细胞裸鼠异种移植瘤的作用及机制
Effects of baicalin on CA46 cell xenografts in nude mice
摘要
Abstract
AIM:To study the effects of baicalin on CA46 cell xenografts in nude mice. METHODS:The nude mice with CA46 cell xenografts were treated with drugs via intraperitoneal injection daily, and were divided into 5 groups; negative control group, 15 mg/kg baicalin group, 30 mg/kg baicalin group, 60 mg/kg baicalin group and 4 mg/kg etoposide ( VP - 16 ) positive control group. After 12 - day treatment, the weight of CA46 cell xenografts stripped from some nude mice in the 5 groups was used to evaluate the effect of baicalin on xenograft growth in the nude mice. The apop-tosis, necrosis and pathological changes of the xenograft cells were examined under light microscope and transmission electronic microscope respectively. The expression levels of phosphatidylinositol 3 - kinase ( PI3K )/protein kinase B ( Akt ) signaling pathway - related proteins extracted from xenografts were determined by Western blotting. The other nude mice with CA46 cell xenografts in the 5 groups continued to be treated with the drugs until death in order to evaluate the effect of baicalin on survival time of the nude mice with CA46 cell xenografts. RESULTS: Baicalin remarkably inhibited the growth of CA46 cell xenografts, induced apoptosis and necrosis of xenograft cells, and reduced the protein expression of phospho - Akt ( p - Akt), nuclear factor - kappa B ( NF - kB ), mammalian target of rapamycin ( mTOR ) and phospho - mTOR ( p -mTOR ) in the xenografts after 12 - day treatment. Furthermore, baicalin prolonged the survival time of the nude mice with CA46 cell xenografts in a dose - dependent manner. CONCLUSION: Baicalin inhibits the growth and induces apoptosis of CA46 cell xenografts in the nude mice, and prolongs the survival time of the nude mice with CA46 cell xenografts through the mechanism of down - regulating PI3K/Akt/NF - kB and PI3K/Akt/ mTOR signaling pathways.关键词
黄芩苷/CA46细胞/裸鼠异种移植瘤/PI3K/Akt信号通路Key words
Baicalin/CA46 cells/Xenografts in nude mice/PBK/Akt signaling pathway分类
医药卫生引用本文复制引用
黄毅,胡建达,郑静,李静,魏天南,郑志红,陈英玉..黄芩苷对CA46细胞裸鼠异种移植瘤的作用及机制[J].中国病理生理杂志,2012,28(1):70-75,6.基金项目
福建省科技创新平台建设项目(No.2009J1004) （No.2009J1004）
福建省高等学校新世纪优秀人才支持计划(No.NCETFJ-0604) （No.NCETFJ-0604）
福建医科大学教授学术发展基金资助项目(No.JS06081) （No.JS06081）
