中国病理生理杂志2012，Vol.28Issue(1)：76-80,5.DOI:10.3969/j.issn.1000-4718.2012.01.014
柴胡皂苷在体外对人白血病细胞株K562/ADM多药耐药性的逆转作用
Reverse effects of saikoside on multidrug resistance of human leukemic cell line K562/ADM in vitro
摘要
Abstract
AIM:To study the reverse effects of saikoside ( SS ) on the multidrug resistance ( MDR ) of human leukemic cell line K562/ADM and to investigate the related mechanism. METHODS:K562 cells and K562/ADM cells in the culture were treated with SS at the concentrations of 1～100 mg/L. The inhibitory rate of the cell proliferation was measured by MTT assay. Non - cytotoxic dose of SS was determined. K562/ADM cells were treated with SS at non - cyto-toxic doses of 1. 25, 2. 5 and 5. 0 mg/L with different concentrations of adriamycin ( ADM,0. 05～100 mg/L ). The 50% inhibitory concentration ( IC50 ) and the reversal index in all groups were determined. The cell morphology was observed after treated with SS + ADM. The effects of SS on ADM accumulation in K562/ADM cells, the cell cycle profile and apopto-sis were examined by flow cytometry. RESULTS: The inhibitory rates were significantly increased in a dose - dependent manner when the cells were treated with different doses of SS ( 1 ～100 mg/L). The available reversal concentration of SS was 5. 0 mg/L and the reversal index was 21. 5 folds for K562/ADM cells. After treated with SS + ADM, the number of tumor cells was decreased and apoptotic cells were increased in a dose - response relationship. ADM accumulation in K562/ADM cells treated with SS was significantly higher than that in control cells ( P <0. 05 ). SS may significantly enhanced the apoptosis of K562/ADM cells treated with ADM ( P < 0. 05 ). K562/ADM cells treated with SS were blocked in the stage of G0/G1. CONCLUSION: SS has effect on proliferation inhibition and MDR reversal in K562/ADM cell line. The reversal mechanisms of SS may be due to increasing the accumulation of chemo therapeutics in the cell, inducing the cell apoptosis and arresting the cells in G0/G1 phase.关键词
柴胡皂苷/多药耐药/耐药逆转/K562/ADM细胞Key words
Saikoside/Multidrug resistance/Reversal of drug resistance/K562/ADM cells分类
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盖晓东,历春,李倩,刘艳波,薛昊罡..柴胡皂苷在体外对人白血病细胞株K562/ADM多药耐药性的逆转作用[J].中国病理生理杂志,2012,28(1):76-80,5.基金项目
吉林省科技发展计划项目(No.200505246) （No.200505246）
