中国病理生理杂志2012，Vol.28Issue(1)：114-117,4.DOI:10.3969/j.issn.1000-4718.2012.01.022
免疫复合物致痛与甲醛致痛大鼠疼痛行为的比较
Comparison of immune-related pain induced by antigen-special complex with inflammatory pain induced by formalin in rats
摘要
Abstract
AIM:To investigate the difference between immune - related pain induced by antigen - special complex and inflammatory pain induced by formalin, and to observe the differential expression of p38 mitogen - activated protein kinase in spinal cord. METHODS:Thirty adult health SD rats were randomly divided into control group, formalin group and immune complex group ( 10 rats in each group ). After the baseline tests were finished, 5 rats in each group underwent intrathecal administration of p38 MAPK inhibitor SB203580. The right hindpaw of the rats were injected with PBS, formalin or rat IgG immune complex. The thickness of hindpaw and pain behaviors were observed at time points of 0 min, 30 min, lh,2h,4h,8h and 12 h after injection. The expression levels of total and activated p38 MAPK in spinal cord were determined by Western blotting analysis. RESULTS: The rats in formalin group showed significant nociceptive behaviors immediately, such as licking foot, and limping with highly swollen foot which could touch the ground. The pain threshold was decreased rapidly 30 min after injection and alleviated after then. The pain threshold of the rats in immune complex group obviously decreased 4 h after injection without red swollen hindpaw. The expression of activated p - p38 MAPK in spinal cord in formalin group was significantly higher than that in immune complex group and control group ( P < 0. 01 ). No statistic difference of p - p38 expression between immune complex group and control group, also no significant effects of SB203580 on pain behaviors in immune complex group were observed. CONCLUSION: Activated p38 MAPK contributes to the pathogenesis of inflammatory pain, but not to the pathogenesis of immune - related pain. The mechanism of immune - related pain is different from inflammatory pain induced by formalin.关键词
炎症性疼痛/免疫相关疼痛/特异性抗原复合物/p38 MAPK通路Key words
Inflammatory pain/Immune-related pain/Antigen-special complex/p38 MAPK pathway分类
医药卫生引用本文复制引用
吴锐,胡文娟,李荣亨..免疫复合物致痛与甲醛致痛大鼠疼痛行为的比较[J].中国病理生理杂志,2012,28(1):114-117,4.基金项目
国家自然科学基金资助项目(No.30960473/H2706) （No.30960473/H2706）
