中国病理生理杂志2012，Vol.28Issue(1)：124-130,7.DOI:10.3969/j.issn.1000-4718.2012.01.024
骨髓间充质干细胞对致敏小鼠造血干/祖细胞移植植入的影响
Effect of bone marrow mesenchymal stem cells on engraftment of hematopoietic stem/progenitor cells in sensitized mice
摘要
Abstract
AIM:To evaluate the effects of bone marrow - derived mesenchymal stem cells ( MSCs ) on en-graftment of hematopoietic stem/progenitor cells in sensitized mice. METHODS:Mouse bone marrow - derived MSCs were cultured by adherent culture method. MSCs combined with or without hematopoietic stem/progenitor cells were implanted into the sensitized mouse model, which was established by allogeneic splenocyte transfusion, and were divided into 6 groups; MSC intervention groups, including sensitized mice with MSCs on day 11 , sensitized mice with MSCs on day 0 and sensitized - mice with MSCs both on day 11 and day 0; control groups, including sensitized mice without MSC intervention, non - sensitized mice without MSC intervention and non - sensitized mice without MSCs or transplantation of hematopoietic stem/progenitor cells. The survivors were assessed after transplantation and hematopoietic recovery was monitored weekly including hematological change, immune function reconstruction, bone marrow cell recovery, chimera analysis and graft - versus - host disease development. RESULTS: Compared with different control groups, MSC intervention did not prolong the survival rates of the sensitized model mice after lethal irradiation. CONCLUSION: Under the experimental conditions, MSC combined with C57BL/6 bone marrow hematopoietic stem/progenitor cells fail to promote the growth of en-graftment in C57BL/6 allogeneic splenocyte -sensitized BALB/c mice in vivo.关键词
骨髓间充质干细胞/致敏小鼠/造血干/祖细胞移植Key words
Bone marrow mesenchymal stem cells/Sensitized mice/Hematopoietic stem/progenitor cell transplantation分类
医药卫生引用本文复制引用
洪冬玲,吴燕峰,许吕宏,方建培..骨髓间充质干细胞对致敏小鼠造血干/祖细胞移植植入的影响[J].中国病理生理杂志,2012,28(1):124-130,7.基金项目
国家自然科学基金资金资助项目(No.30872383) （No.30872383）
