中国病理生理杂志2012，Vol.28Issue(1)：131-135,5.DOI:10.3969/j.issn.1000-4718.2012.01.025
转染isl1基因促进骨髓间充质干细胞向心肌样细胞分化
isl1 gene transfection promotes BMSCs to diffenentiate into cardiomyocytes
摘要
Abstract
AIM:To investigate the role of Isll protein in the differentiation of bone marrow mesenchymal stem cells ( BMSCs ) into cardiomyocyte in cardiac micro environment. METHODS:BMSC - isll stable cell line was constructed using lentivirus vector. BMSCs, BMSC - vehicle and BMSC - isll with neonatal rat cardiomyocytes were co - cultured in a ratio of 1:10 and separated by Transwell chambers for 1 week. The expression profiles of the myocardial intra - markers were tested to determine the differentiation efficiency of BMSC - isll stable cell line by real - time PCR, Western blotting and immunofluorescence analysis. RESULTS: A BMSC - isll stable cell line was obtained. After co - cultured for 1 week, the results of real -time PCR demonstrated that the expression levels of GATA binding protein 4 ( GATA4 ), NK2 transcription factor related, locus 5 ( Nkx2. 5 ), myocyte enhancer factor 2C ( MEF2C ) and ryanodine receptor 2 ( RyR2 ) were higher (2.3 - ,2.7 - ,2.6- and 3. 2 -fold, respectively ) in BMSC - isll stable cell line than those in BMSCs and BMSC - vehicle. The results of Western blotting and immunofluorescence showed that the expression levels of cardiac tropo-nin T ( cTnT ), cardiac troponin I ( cTnl) and a - actinin significantly increased in BMSC - isll compared with BMSCs and BMSC -vehicle. CONCLUSION: The differentiation efficiency of BMSCs into cardiomyocytes is higher in BMSC - isll stable cell line than that in the primary BMSCs.关键词
骨髓间充质干细胞/慢病毒/Isli蛋白/微环境/心肌样细胞Key words
Bone marrow mesenchymal stem cells/Lentivirus/Isll protein/Microenvironment/Myocardium-like cells分类
医药卫生引用本文复制引用
李杏肖,余细勇,李晓红,林秋雄,肖定璋,刘晓颖,单志新,朱杰宁,麦丽萍,夏慧苏..转染isl1基因促进骨髓间充质干细胞向心肌样细胞分化[J].中国病理生理杂志,2012,28(1):131-135,5.基金项目
国家自然科学基金重大国际合作项目(No.81120108003) （No.81120108003）
国家自然科学青年基金资助项目(No.30900610) （No.30900610）
广东省自然科学基金资助项目(No.9451008002003467) （No.9451008002003467）
