中国神经再生研究（英文版）2012，Vol.7Issue(2)：85-90,6.DOI:10.3969/j.issn.1673-5374.2012.02.001
Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha- synuclein
Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha- synuclein
Tao Chen 1Xiaoping Liao 1Guoqiang Wen 1Yidong Deng 1Min Guo 1Zhigang Long 1Feng Ouyang1
作者信息
- 1. Department of Neurology, Hainan Provincial People's Hospital, Haikou 570311, Hainan Province, China
- 折叠
摘要
Abstract
The specific and effective α-synuclein RNA interference (RNAi) plasmids, and the α-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 (HEK293) cells using the lipofectamine method. Using an inverted fluorescence microscope, α-synuclein proteins were observed to aggregate in the cytoplasm and nucleus. Wild-type α-synuclein proteins co-localized with mitochondria. Hematoxylin-eosin staining revealed round eosinophilic bodies (Lewy body-like inclusions) in the cytoplasm of some cells transfected with α-synuclein-pEGFP plasmid. However, the formation of Lewy body-like inclusions was not observed following transfection with the RNAi pSYN-1 plasmid. RNAi blocked Lewy body-like inclusions in the cytoplasm of HEK293 cells induced by wild-type α-synuclein overexpression, but RNAi did not affect the subcellular localization of wild-type α-synuclein in mitochondria.关键词
RNA interference/α-synuclein gene/subcellular localization/inclusion/Parkinson's disease/neural regenerationKey words
RNA interference/α-synuclein gene/subcellular localization/inclusion/Parkinson's disease/neural regeneration引用本文复制引用
Tao Chen,Xiaoping Liao,Guoqiang Wen,Yidong Deng,Min Guo,Zhigang Long,Feng Ouyang..Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alpha- synuclein[J].中国神经再生研究（英文版）,2012,7(2):85-90,6.
