广东医学2012，Vol.33Issue(1)：40-43,4.
非酒精性脂肪性肝炎大鼠肝细胞和库普弗细胞同时分离及鉴定
Simultaneously isolation and identification of hepatocytes and kupffer cells from nonalcohlic steatohepatitis rat
摘要
Abstract
Objective To establish an effective and stable method for isolation and identification of hepatocytes ( HCs ) and Kupffer cells ( KCs ) from nonalcohlic steatohepatitis ( NASH ) rats simultaneously. Methods Rat liver in vitro was perfused with collagenase IV and centrifuged in low - speed isolation of HCs. Subsequently, differential centrifu-gation, density gradient centrifugation and selective adherence were carried out for isolation of KCs. Typan blue stain and Flow cytometry ( FCM ) were applied for tests of activity and purity. Results The yields of purified cell of HC and KC in each rat were ( 4. 0 ~4. 5 ) x 108 and ( 1. 5 ~2. 0 ) x 107 , respectively. The viability of HCs and KCs isolated were higher than 95% , with purity over 90%. Conclusion The method for isolation and identification of HCs and KCs from NASH rat simultaneously is effective and stable. Both quantity and purity are qualified for further experiments.关键词
非酒精性脂肪性肝炎大鼠/肝细胞/库普弗细胞/同时分离/鉴定Key words
NASH rats/HCs and KCs/simultaneous isolation/identification引用本文复制引用
冯高飞,杨晓蕾,杨钦河,王文晶,何秀敏,张玉佩,纪桂元,李光秋,赵亚凤,沈灿宏..非酒精性脂肪性肝炎大鼠肝细胞和库普弗细胞同时分离及鉴定[J].广东医学,2012,33(1):40-43,4.基金项目
国家自然科学基金资助项目(编号:30973694),国家大学生创新性实验计划资助项目(编号:091055930) （编号:30973694）
