广东医学2012，Vol.33Issue(1)：44-48,5.
神经生长因子诱导神经干细胞分化的作用机制探讨
Mechanisms of NGF-induced differentiation in neural stem cells
摘要
Abstract
Objective To study the effects of NGF on the differentiation of neural stem cells ( NSCs ) and the underlying mechanisms. Methods NSCs were isolated from E 13 ~ 14 d Kunming mice and cultured in serum - free medium. After three passages, NSCs were treated with NGF and differentiated neurons were identified by immunocytochemistry with MAP - 2 antibody. The number of MAP - 2 positive cells was assessed using microscope, while NGF - induced phos-phorylation of TrkA, Akt and Erk was determined by Western blot. To study the signaling pathway involved, NSCs were pretreated with various pathway inhibitors and subsequently with NGF. Immunocytochemistry and Western blot were used to investigate the role of each pathway on the differentiation of NSCs induced by NGF. Results NGF promoted the differentiation of NSCs into neurons and increased the phosphorylation of TrkA, Akt and Erk in time - and dose - dependent manners. The effect of NGF was blocked by the PDK/Akt pathway inhibitor LY294002 and the Akt inhibitor Akt VIII, but not by Erk inhibitors. Conclusion NGF induces the differentiation of NSCs into neurons through the activation of PDK/Akt signaling pathways.关键词
神经干细胞/神经分化/神经生长因子/信号通路Key words
neural stem cells/differentiation/nerve growth factor/signaling pathway引用本文复制引用
苏肖英,汪海涛,孟茜,Remi Quirion,郑文华..神经生长因子诱导神经干细胞分化的作用机制探讨[J].广东医学,2012,33(1):44-48,5.基金项目
国家自然科学基金资助项目(编号:30670652,30711120565) （编号:30670652,30711120565）
