军事医学2012，Vol.36Issue(1)：24-26,3.
紫外线通过诱导TNF-α胞内聚集间接调控VEGF分泌表达
Intracellular accumulation of TNF-α is involved in VEGF induction upon ultraviolet B irradiation
摘要
Abstract
Objective To explore the role of TNF-ct in mediating VEGF expression under UVB exposure. Methods The expression plasmid containing TNF-α short hairpin RNA( shRNA ) was introduced into the mouse embryonic fibroblasts ( MEFs ) and then the stable transfectants were established. The efficiency of TNF-α shRNA in blocking TNF-α induction under UVB irradiation was confirmed by RT-PCR assay. The transcription of vegf under UVB irradiation was determined by the luciferase reporter assay. The secretion of VEGF induced by UVB was determined by ELISA. Results TNF-α shRNA efficiently blocked UVB-induced TNF-α transcription. Under the same condition, VEGF transcription and expression were significantly inhibited. Conclusion TNF-ct induction is involved in VEGF expression in the cellular UVB response.关键词
紫外线/TNF-α/VEGFKey words
UVB/ TNF α/ VEGF分类
生物科学引用本文复制引用
胡美茹,董雯,李译,买三月,李小光,郭宁,宋伦..紫外线通过诱导TNF-α胞内聚集间接调控VEGF分泌表达[J].军事医学,2012,36(1):24-26,3.基金项目
国家自然科学基金资助项目(30871277,30970594,31171342) （30871277,30970594,31171342）
北京市自然科学基金资助项目(5092022,5102035) （5092022,5102035）
国家重点基础研究发展规划资助项目(2011CB503803) （2011CB503803）
