畜牧兽医学报2011，Vol.42Issue(1)：65-70,6.
羊细粒棘球蚴病抗体间接ELISA检测方法的建立
Development of an Indirect ELISA for Detection of Sheep Antibodies against Echinococcosis granulosa
摘要
Abstract
indirect ELISA showed that: HIS-lEG95s was better than GST-lEG95s as the coating antigen. The determine criteria of the indirect ELISA were: Samples with an OD450nm value ≥0. 235 were judged as positive, and with an OD150nmvalue≤0.191 were judged as negative, while with an OD450nm value ranging between them was suspicious. 70 positive and negative serum of sheep collected in Xinjiang with or without Echinococcosis granulosa were detected by the indirect ELISA respectively, and the results showed that this indirect ELISA method was completely consistent with the indirect ELISA provided by Wallaceville Animal Research Centre of New Zealand. There were no cross reactivity detected with other proteins. The variant coefficient in batch and between batches varied from 3,8% to 5.6% and from 5.7% to 8.5%, respectively. All these results showed that the indirect ELISA possessed specificity, sensitivity and good reproducibility. The indirect ELISA for detection of sheep antibodies against Eckiriococcosis granutosa were successfully developed, which provide a fast and simple means, and can be used for clinical detection.关键词
羊细粒棘球蚴病/EG95s/重组融合蛋白/间接ELISA/检测Key words
Echinococcosis granulosa of sheep/EG9Ss/recombinant fusion protein/ indirect ELISA/detection分类
畜牧业引用本文复制引用
贾红,刘丹,侯绍华,于琳琳,柏丽华,朱鸿飞..羊细粒棘球蚴病抗体间接ELISA检测方法的建立[J].畜牧兽医学报,2011,42(1):65-70,6.基金项目
国家科技支撑计划(2008BAK51B08) （2008BAK51B08）
