安徽中医学院学报2012，Vol.31Issue(1)：34-38,5.DOI:10.3969/j.issn.1000-2219.2012.01.011
芪黄煎剂对胃切除大鼠小肠黏膜上皮内和固有层T淋巴细胞亚群的影响
Effects of Qihuang Decoction on T-cells Subsets of Intraepithelial Lymphocytes and Lamina Propria Pymphocytes in Small Intestinal Mucosa of Rats with Stomach Excision
摘要
Abstract
Objective To observe the effects of Qihuang Decoction,a traditional Chinese medicine prescription mainly composed of Astragalus mongholicus and Rhubarb for invigorating spleen-qi and purgation, on T cells subsets of intraepithelial lymphocytes (IELs ) and lamina propria lymphocytes (LPLs) in rats after stomach excision.Methods Sixty rats were randomly divided into sham operation group,model group, and Qihuang Decoction group. The rats in the sham operation group were only treated with an operation of abdominal midline incision and suture with the stomach retaining. The rats in the model group were treated with an operation of stomach excision and Nutrison (an intacted protein enteral nutrition powder) after operation. The rats in the Qihuang Decoction group were given Nutrison and Qihuang Decoction after an operation of stomach excision. The treatment course was one week. At the end of the course,IELs and LPLs in small intestinal mucosa were isolated. The T-cells subsets including αβT cell antigen receptor (αβTCR)-cluster of differentiation 3 positive (CD3+) ,cluster of differentiation 4 positive (CD4+) and cluster of differentiation 8 positive (CD8+), were detected by using flow cytometry.Results (1)One week after the operation, the proportions of αβTCR-CD3+, CD4+, CD8+ T-cells of IELs were significantly decreased as compared with those in the sham operation group (P＜0. 05, or P＜0. 01) ; compared with those in the model group,the proportions of CD3+,CD8+ of IELs were significantly increased (P＜0. 01).(2)Compared with those in the sham operation group,the proportions of αβTCR-CD3+, CD4+, CD8+ of LPLs were significantly decreased ( P＜0. 01) ;while compared with those in the model group,the proportions of αβTCR-CD3+,CD4+ in Qihuang Decoction group were significantly increased(P＜0. 05). Conclusion Qihuang Decoction can promote the maturation,differentiation,and multiplication of IELs and LPLs,which is benefit for the regulation and recovery of intestinal immune barrier after the stress of stomach excision.关键词
胃切除/肠黏膜免疫屏障/上皮内淋巴细胞/固有层淋巴细胞/芪黄煎剂Key words
stomach excision/ intestinal mucosa barrier/ intraepithelial lymphocytes/ lamina propria lym phocytes / Qihuang Decoction分类
医药卫生引用本文复制引用
袁以洋,于庆生,潘晋方,帅剑锋,张皖东,张琦,刘举达,张千一..芪黄煎剂对胃切除大鼠小肠黏膜上皮内和固有层T淋巴细胞亚群的影响 [J].安徽中医学院学报,2012,31(1):34-38,5.基金项目
国家自然科学基金(30973855) （30973855）
