畜牧兽医学报2011，Vol.42Issue(1)：33-38,6.
鸭Akirin2基因克隆、序列分析与组织表达特性研究
Cloning,Sequence Analysis and Specific Expression in Different Tissues of Duck Akirin2 Gene
摘要
Abstract
In order to understand the function of Akirin2 gene in duck, the duck AkirinZ gene was cloned by RT-PCR, and the relative mRNA expression of AkirinZ was detected in different tissues using the qPCR method. The results of the sequence analysis showed that the CDS region of the duck AkirinZ gene included 594 nucleotides, encoding for 197 amino acids, and the amino acid homology of Akirin2 between the duck and the chicken was 99%. The secondary structure of Akirin2 amino acids had a common domain with the mammals Akirinl and Akirin2. The results of the qRT-PCR indicated that the duck Akirin2 express in the muscle and the immune organ spleen. These results supported the previously views that duck AkirinZ gene had a common function with the Akirinl and AkirinZ genes in mammals, and these assays made a foundation work for the further research about the AkirinZ gene in myogenesis in birds.关键词
鸭/Akirin2/序列分析/荧光定量PCRKey words
duck/ Akirin2/ sequence analysis/ qPCR分类
农业科技引用本文复制引用
代飞,黄锎靓,刘贺贺,韩春春,李亮,王继文..鸭Akirin2基因克隆、序列分析与组织表达特性研究[J].畜牧兽医学报,2011,42(1):33-38,6.基金项目
国家科技支撑计划重点项目(2008BADB2B08) （2008BADB2B08）
国家大学生创新性实验计划项目(081062610) （081062610）
