畜牧兽医学报2011，Vol.42Issue(1)：48-55,8.
猪细小病毒感染PK-15细胞抗病毒相关因子转录变化的分析
The Analysis of Antiviral Cytokines Transcriptional Profiles of PK-15 Cell Cultures Following Infection with Porcine Parvovirus
摘要
Abstract
In order to survey the host inflammatory responses, particularly the antiviral cytokines responses of Porcine Parvovirus (PPV) infection, and to investigate the host-PPV interaction, Porcine Kidney-15 cells (PK-15) were infected by PPV. Then the viral DNA was measured and analyzed using real-time PCR. The transcript level of cytokines (IFN-β, IFN-y, IFNAR1, IF-NAR-2, MHC-I , MHC-Ⅱ , MX1, NOS, 2-5AS, Rnase L and IRF-3) were detected by realtime PCR too. The results showed that PPV proliferated rapidly in PK-15 cell 12 hours after infection, and reached peak 48 hours after infection; the transcription levels of IFN-β, IFN-y, IFNAR-1, IFNAR-2, MHC- I , MHC- Ⅱ , MX1, iNOS, 2-5AS, Rnase L and IRF-3 were increased obviously, and the transcription level of Mxl gene was increased 8 423 times at 24 hours after infection. The results revealed that the level of antiviral cytokines increased in PPV infected PK-15 cell.关键词
猪细小病毒/细胞因子/PK-15细胞/转录时相Key words
porcine parvovirus/ cytokines/ PK-15 cell/ transcriptional profiles分类
农业科技引用本文复制引用
李厚伟,魏战勇,尹海燕,陈红英,李金磊,韩志涛,崔保安..猪细小病毒感染PK-15细胞抗病毒相关因子转录变化的分析[J].畜牧兽医学报,2011,42(1):48-55,8.基金项目
河南省杰出人才创新基金项目(0621002100) （0621002100）
