安徽中医学院学报2012，Vol.31Issue(1)：43-47,5.DOI:10.3969/j.issn.1000-2219.2012.01.013
桑色素对脑缺血再灌注损伤大鼠多聚腺苷酸二磷酸核糖聚合酶和核因子-κB表达的影响
Effects of Morin on Expression of Poly Adenosine Diphosphate Ribose Polymerase and Nuclear Factor-κB in Rats Undergone Cerebral Ischemia-Reperfusion Injury
摘要
Abstract
Objective To investigate the effects of morin on the expression of poly adenosine diphosphate ribose polymerase (PARP) and nuclear factor-κB (NF-κB), both of which are inflammatory transmitters,in rats undergone cerebral ischemia-reperfusion injury. Methods The model of ischemia reperfusion injury of middle cerebral artery was induced by suture embolism method. Rats were randomly divided into sham operation group, model group, nimodipine group, and low-, middle- and high-dose morin groups. The neurological function was investigated by neurological severity scores (NSS) , and the expressions of PARP and NF-κB protein and their mRNA in infarction region were detected by using western blot and quantitative real-time fluorescence quantitative polymerase chain reaction (RT-PCR), respectively. Results Compared with those in the model group, the neurological scores (NSS) of low-, middle- and high-dose morin groups were significantly decreased (P＜0. 01) ; the expressions of PARP protein, NF-κB protein, PARP mRNA,and NF-κB mRNA in penumbral area were also significantly decreased (P＜0. 05,or P＜0. 01).The effects of high-dose morin were significantly superior to those of low- and middle-dose morin as well as nimodipine in decreasing the levels of above-mentioned indexes (P＜0. 05,or P＜0. 01). Conclusion Morin can inhibit inflammatory reaction by down regulating the expressions of PARP and NF-κB; consequently, it promotes the recovery of damaged nerve function induced by cerebral ischemia injury.关键词
桑色素/脑缺血再灌注损伤/核因子-κB/多聚腺苷酸二磷酸核糖聚合酶Key words
morin/ cerebral ischemia-reperfusion/ nuclear factor-κB/ poly adenosine diphosphate ribose polymerase分类
医药卫生引用本文复制引用
王莹,丁利,李文媛,管业秋,赵斯达,张秀彪,吕文召..桑色素对脑缺血再灌注损伤大鼠多聚腺苷酸二磷酸核糖聚合酶和核因子-κB表达的影响 [J].安徽中医学院学报,2012,31(1):43-47,5.基金项目
黑龙江省普通高等学校青年学术骨干支持计划项目(1152G050) （1152G050）
黑龙江省卫生厅科研项目(2010243) （2010243）
