安徽中医学院学报2012，Vol.31Issue(1)：73-77,5.DOI:10.3969/j.issn.1000-2219.2012.01.022
总姜黄素3种单体高纯度同时分离方法
Simultaneous Separation of Three High Purity Monomers from Curcuminoids
摘要
Abstract
Objective To investigate a technology for separating and purifying three monomers from curcuminoids, including curcumin (Cur Ⅰ ), demethoxycurcumin (Cur Ⅱ ), and bisdemethoxycurcumin (Cur Ⅲ). Methods Three monomers were separated from curcuminoids by column chromatography with chloroform-methanol solvent system for gradient elution, and purified with recrystallization. Threee monomers of curcuminoids were detected and their purities were calculated by thin-layer chromatography (TLC ), melting point determination, and high performance liquid chromatography ( HPLC ).Results Three high purity curcumin monomers were gained in this experiment, and their purities were Identified by HPLC method. The purity of Cur Ⅰ, Cur Ⅱ, and Cur Ⅲ was 99. 47%, 99. 64%, and 98. 64%, respectively. Conclusion Silica gel column chromatography can provide an experimental basis for the development and application of curcumin monomers because of its being reasonable, scientific and high efficient.关键词
姜黄素/脱甲氧基姜黄素/双脱甲氧基姜黄素/柱层析Key words
curcumin/ demethoxycurcumin/ bisdemethoxycurcumin/ column chromatography分类
中医学引用本文复制引用
秦晓燕,龚菊梅,陈卫东..总姜黄素3种单体高纯度同时分离方法[J].安徽中医学院学报,2012,31(1):73-77,5.基金项目
安徽中医学院引进人才科研基金(2009rc001) （2009rc001）
