感染、炎症、修复2011，Vol.12Issue(4)：213-216,4.
电针足三里穴对失血性休克延迟补液大鼠肠组织缺血性损伤的影响
Effects of electro-acupuncture of Zusanli points(足三里穴)on ischemia injury of gut in rats with delayed fluid replacement after hemorrhagic shock
摘要
Abstract
To investigate the effects of electro-acupuncture of Zusanli points (足三里穴)on ische mia inj ury to gut in rats with delayed fluid replacement after hemorrhagic shock. Methods: Thirty SD male rats with 40% blood loss were randomly divided into 3 groups (n =10) : immediate fluid resuscitation (IFR) group, delayed fluid resuscitation with sham electro-acupuncture group (SEA/DFR group) , and delayed fluid resuscitation with electro-acupuncture Zusanli points group (EA/DFR group). IFR group received fluid replacement with Ringer's solution in volume 2 times of lost blood 10 minutes after blood loss. Electro-acupuncture was given to bilateral Zusanli points (ST36) with constant voltage (4 Hz, 4 V ) for 25 minutes approximately 10 minutes after blood loss, while no-acupuncture was conducted in SEA/DFR group. Two EA groups received delayed fluid resuscitation 3 hours after blood loss. The intestinal mucosal blood flow (IMBF) and diamine oxidase (DAO)were measured before hemorrhage, 3 hours and 24 hours after hemorrhage. The tissue water content of jejunum was also determined 12 hours after hemorrhage. Results;The levels of IMBF and DAO in gut tissue were significantly decreased, the rate of tissue water content obviously increased after blood loss in all groups (all P<0. 05). Three hours after blood loss, IMBF and DAO levels of EA/DFR group and IFR group were significantly higher than those of SEA/DFR group, while IMBF and DAO of EA/DFR group were significantly lower than those of IFR group(all P<0. 05). Twelve hours after blood loss, IMBF and DAO levels were higher and the rate of tissue water content was lower in EA/DFR group and IFR group compared with those of the SEA/DFR group, IMBF and the rate of tissue water content of EA/DFR group showed no significant difference compared with those of IFR group (both P<0. 05) , while DAO levels of EA/DFR group were lower than that of IFR group(P<0. 05).Conclusions: The results suggest that electroacupuncture of Zusanli points alleviates gut ischemia injury in rats with delayed fluid resuscitation after hemorrhagic shock.关键词
休克,失血性/足三里穴/电针/肠/二胺氧化酶/局部血流量Key words
Hemorrhage shock /Zusanli point/Electro-acupuncture/Gut Diamine oxidase /Intestinal mucosal blood flow引用本文复制引用
钟毓贤,石现,赵莹,刘维维,姚甲瑞,张慧苹,胡森..电针足三里穴对失血性休克延迟补液大鼠肠组织缺血性损伤的影响[J].感染、炎症、修复,2011,12(4):213-216,4.基金项目
全军医学科研"十一五"专项课题(06Z055) （06Z055）
