畜牧兽医学报2011，Vol.42Issue(1)：141-144,4.
牦牛胚胎性别鉴定PCR反应体系的建立
Sexing of in vitro-produced Yak Embryos by Nested-PCR
摘要
Abstract
The objective of this study was to obtain an accurate and reliable method for determining the sex of yak embryos through amplification of SRY gene of the yaks, and evaluate embryonic development after biopsies. Two pairs of SRY gene nested primers for sex determination and two pairs of HSL gene primers as internal standard and optimized PCR reaction conditions were designed. The accuracy of PCR amplification for sex determination was verified by 24 blood samples. The result showed that in every case, the results were as expected for both female and male, I. E. , accuracy was 100%. Using this optimized procedure, clear signals following PCR amplification were obtained from all samples of IVF-derived yak embryos, eight cells were sampled from each embryo, 45. 8% (11/24) embryos for males and 54. 2% (13/24) for females. 56. 7% (17/30) of biopsied embryos could further develop. It was concluded that this PCR system was highly reliable for sex determination of yak embryos.关键词
牦牛/体外受精/胚胎/巢式PCR/性别鉴定Key words
yak/in vitro fertilization/embryo/nested-PCR/sex determination分类
畜牧业引用本文复制引用
张大伟,字向东,黄磊,马力,陈达文,徐华伟,梁冠男..牦牛胚胎性别鉴定PCR反应体系的建立[J].畜牧兽医学报,2011,42(1):141-144,4.基金项目
四川省科技厅应用基础项目(07JY029-128) （07JY029-128）
四川省学术带头人培养基金资助 （）
