畜牧兽医学报2011，Vol.42Issue(1)：1-7,7.
阉割对猪甲状腺转录因子-1、2(TTF-1,2)基因表达的影响
Influence of Castration on Porcine Thyroid Transcription Factor-1,2(TTF-1,2) Gene Expression
摘要
Abstract
In order to investigate the effect of castration on expression of TTF-1 and TTF-2 genes, real-time fluorescence quantitative PCR was applied to investigate the developmental expression patterns of TTF-1 and TTF-2 genes from the thyroid tissue of castrated and uncastrated Jinhua pigs on the age of 60, 90 and 120 days. The results showed that the expression of TTF-1 and TTF-2 genes increased slightly in uncastrated pigs, while they were relatively stable in castrated pigs. The expression level of TTF-1 and TTF-2 mRNA in castrated Jinhua pigs on 60 and 90 days were higher than that of uncastrated pigs, while the expression level of TTF-1 and TTF2 on 120 days were lower than that of uncastrated pigs, but no significant difference (PX). 05). The results indicated that the real-time PCR method was faithful for detecting the expression analysis of TTF-1 and TTF-2 genes, and the expression of TTF-1 and TTF-2 mRNA increased after castration. Furthermore, there was a significant correlation between TTF-1 gene expression and meat quality traits.关键词
猪/阉割/荧光定量PCR/甲状腺转录因子-1(TTF-1)/甲状腺转录因子-2(TTF-2)Key words
pig/castration/real-time PCR/thyroid transcription factor 1 (TTF-1)/thyroid transcription factor 2 (TTF-2)分类
农业科技引用本文复制引用
王颖,张立凡,蔡兆伟,陈哲,蒋晓玲,周红梅,华绪川,徐宁迎..阉割对猪甲状腺转录因子-1、2(TTF-1,2)基因表达的影响[J].畜牧兽医学报,2011,42(1):1-7,7.基金项目
国家重点基础研究发展规划项目(2006CB102100) （2006CB102100）
国家自然科学基金项目(30972078) （30972078）
