感染、炎症、修复2011，Vol.12Issue(4)：202-204,封2,4.
汗腺样细胞经冻存复苏后再生汗腺功能的研究
Study on the sudation function of sweat gland-like cells differentiated from umbilical cord mesenchymal stem cells after being frozen and defrosted
摘要
Abstract
To explore the biological characteristics and the function of sweat gland-like cells trans-differentiated from umbilical cord mcscnchymal stem cells (UCMSCs) after being deep freeze storage. Methods; Human UCMSCs and sweat gland cells were isolated, cultured and identified with specific markers. They were co-cultured to transform the UCMSCs to sweat gland-like cells. After being deep frozen and defrosted, the stem cells, which exhibited the phenotype of sweat gland cells, were implanted into scald injured paws of nude mice. The regeneration of the sweat gland cells was observed. Results;The transdiffcrcntiatcd sweat gland-like cells were successfully implanted into the scald paws of nude mice after being frozen and defrosted, which regenerated the function of perspiration. Pathological observation showed that sweat glands were regenerated. Conclusions: UCMSCs can be transdiffcrcntiatcd into sweat gland-like cells in vitro, and the deep frozen and defrosted sweat gland like cells can be implanted into a fresh wound to regenerate sweat glands.关键词
脐带间充质干细胞/汗腺/再生Key words
Umbilical cord mcscnchymal stem cells Sweat glands Regeneration引用本文复制引用
马奎,鲁刚,杨思明,孙同柱,付小兵,盛志勇..汗腺样细胞经冻存复苏后再生汗腺功能的研究[J].感染、炎症、修复,2011,12(4):202-204,封2,4.基金项目
国家973计划资助项目(2012CB518105) （2012CB518105）
国家自然科学基金(81121004,81000843) （81121004,81000843）
