农业机械学报2011，Vol.42Issue(4)：127-132,138,7.
酶法转化糖苷型沙棘叶黄酮生成苷元工艺优化
Optimization of Enzymatic Conversion of Flavonoids Extracted from Sea Buckthorn Leaves
摘要
Abstract
In order to increase the yield of sea buckthorn leaves flavonoid aglycones converted from flavonoid glycosides by the enzyme-Pectinex Ultra SP-L, on the basis of single-factor test, response surface analysis method was used. Hydrolysis time, hydrolysis temperature and pH value were chosen to be independent variables. The value of flavonoid aglycones quality was regarded as the response value. The results showed that the hydrolysis time was a significant effect factor and hydrolysis temperature and pH value had no significant impact. The optimum conditions for enzymatic conversion were hydrolysis temperature of 50. 8℃ , pH value of 5. 16, hydrolysis time of 2.5 h. Under those conditions, the total aglycones quality obtained from 15 mg sea buckthorn leaf extract was 0. 180 1 mg and the conversion rate to flavonoids aglycones was 62% .关键词
聚半乳糖醛酸酶/沙棘叶/黄酮/响应面/转化率Key words
Pectinex Ultra SP-L/ Sea buckthorn leaves/ Flavonoids/ Response surface/Conversion rate分类
轻工纺织引用本文复制引用
冯淑环,殷丽君,袁蕊,韭泽悟,李刚,程永强..酶法转化糖苷型沙棘叶黄酮生成苷元工艺优化[J].农业机械学报,2011,42(4):127-132,138,7.基金项目
国家自然科学基金资助项目(30972286)和"十一五"国家科技支撑计划资助项目(2007BAD83B03) （30972286）
