中国药理学通报2012，Vol.28Issue(2)：208-212,5.DOI:10.3969/j.issn.1001-1978.2012.02.014
黄芩苷对FM1肺炎小鼠肺损伤的作用机制研究
Mechanism of Baicalin on lung tissue injuryof mice with FM1 induced pneumonia
摘要
Abstract
Aim To explore mechanism of BAI on lung tissue injury of mice with FM1 induced pneumonia. Methods 96 ICR mice were randomly divided into normal group, model group, RBVgroup (100 mg · kg-1 · d-1 ), BAI groups ( 1500,750,375 mg · kg-1 · d-1 ),with 16 mice in each group. The model of mouse influenza virus pneumonia was prepared by infection of influenza virus strain A/FM/l/47( H1N1 ). Model mice were treated with different doses of BAI by intragastric administration. The pathological change of lung injury was observed; RT-PCR was adopted to determine the mRNA expressions of c-jun and c-fos; Western blot was used to determine the protein expressions of c-jun and p-c-jun; ELISA was applied to test the inflammatory cytokines in lung tissue homogenate.Results Compared with the model, BAI, at doses 750 mg ·kg-1 and 375 mg·kg-1, obviously alleviated the pathological change of lung injury, significantly down-regulated mRNA expressions of c-jun and c-fos ( P < 0. 05 , P < 0. 01 ), significantly downregulated protein expressions of c-jun and p-c-jun( P < 0. 01 )and also inhibited secretions of TNF-α and IL-1 β( P < 0. 01 ). Conclusions BAI can alleviate inflammatory pathological injury induced by FM1. It may inhibit the high expression of transcription factor AP-1 induced by influenza virus infection to decrease the secretion of inflammatory cytokines, and plays the function of anti-influenza virus infection.关键词
黄芩苷/甲型流感病毒FM1/肺炎/c-jun/c-fos/RT-PCR/Western/blotKey words
Baicalin/ influenza virus FM1/ pneumonia/c-jun/ c-fos/ RT-PCR/ Western blot分类
医药卫生引用本文复制引用
万巧凤,顾立刚,殷胜骏,吴珺 ,邱泽计..黄芩苷对FM1肺炎小鼠肺损伤的作用机制研究[J].中国药理学通报,2012,28(2):208-212,5.基金项目
国家自然科学基金资助项目(No 30772872) （No 30772872）
