实用医学杂志2012，Vol.28Issue(2)：181-183,3.DOI:10.3969/j.issn.1006-5725.2012.2.007
抑制PI3K/Akt信号通路提高膀胱癌化疗效果的实验研究
Inhibition of PI3K/Akt signal pathway improves chemotherapeutic effect of bladder cancer
摘要
Abstract
Objective To explore whether the inhibition of PI3K/Akt signal pathway increases the tumor-killing effects of anticancer drugs paclitaxel on bladder cancer T-24 cells. Methods The inhibitory rate of T-24 cells were observed by MTT assay after treatment with different concentrations of individual Akt inhibitor deguelin, paclitaxel, and deguelin plus paclitaxel, and their cell cycle were detected by flow cytometry. Results The inhibition ratio of proliferation of T-24 cells was increased significantly by Paclitaxel in combination with deguelin (P < 0.01). Deguelin had synergistic effects with Paclitaxel (combination index < 1); Flow cytometry showed that deguelin and Paclitaxel blocked the cell cycle mainly in the G0/G1 stage, while the combined medication lengthen the G0/G1 phase and shorten the S phase compared with individual deguelin or Paclitaxel. Conclusion The tumor-killing effect of Paclitaxel on T-24 cells was increased significantly by inhibition of PI3K/Akt signal transduction pathway.关键词
药物协同作用/鱼藤素/P13K/Akt/T-24细胞Key words
Drug synergism/ Deguelin/ PI3K/Akt/ T-24 cell引用本文复制引用
董自强,江克华,宋兴福,陈先国,陈晓波,袁红纲,刘幼昆,龙兵..抑制PI3K/Akt信号通路提高膀胱癌化疗效果的实验研究[J].实用医学杂志,2012,28(2):181-183,3.基金项目
三峡大学硕士学位论文培优基金项目(编号:2011PY031) （编号:2011PY031）
三峡大学研究生科研创新基金项目(编号:2010CX052) （编号:2010CX052）
