中国药理学通报2012，Vol.28Issue(2)：266-269,4.DOI:10.3969/j.issn.1001-1978.2012.02.026
吡格列酮对脂多糖刺激的星形胶质细胞白介素-1β的抑制作用及机制
Effects of pioglitazone on the expression of interleukin-1β induced by lipopolysaccharide in cultured astrocytes in rats and signaling mechanisms
摘要
Abstract
Aim To investigate whether pioglitazone could protect cortical astrocytes from LPS-induced expressions of IL-1 β and the mechanisms responsible for this protective effect. Methods Cultured astrocytes were treated with LPS (10 mg · L-1 ) for 24 h, in the absence or presence of pioglitazone ( 0. 1, 1.0 and 10. 0 μmol· L-1 ) or the specific inhibitor of JNK SP600125 ( 10. 0 μmol · L-1 ), and harvested. IL-1β was analysed by ELISA and immunofluorescent technology. Western blot was performed to observe the level of p-JNK and p-c-Jun in cultured astrocytes. Results LPS significantly induced increase in phospho-JNKl, phospho-c-Jun protein expression without affecting total protein levels and increase the expression and production of IL-1 β in cultured astrocytes in rats. Pioglitazone inhibited LPS-induced increase in expression of IL-1 β, and p-JNK and p-c-Jun in cultured astrocytes. SP600125 significantly inhibited LPS-induced increase in production of IL-1 (3. Conclusion This study indicates that pioglitazone can inhibit the expression of IL-1(3 induced by LPS in cultured astrocytes through the suppression of JNK signal transduction pathway activity.关键词
吡格列酮/脂多糖/星形胶质细胞/白介素-1β/c-Jun氨基末端激酶/转录因子c-JunKey words
pioglitazone/ lipopolysaccharide/ astrocytes / interleukin-1β/ c-Jun N-terminal kinases/ c-Jun分类
医药卫生引用本文复制引用
刘卓,隋海娟,闫恩志,刘婉珠,金英..吡格列酮对脂多糖刺激的星形胶质细胞白介素-1β的抑制作用及机制[J].中国药理学通报,2012,28(2):266-269,4.基金项目
辽宁省教育厅创新团队项目(No LT2010064) （No LT2010064）
辽宁医学院院内资助课题(No Y2010Z003) （No Y2010Z003）
