中国药理学通报2012，Vol.28Issue(2)：286-289,4.DOI:10.3969/j.issn.1001-1978.2012.02.031
黄芩提取物中黄酮类成分血浆蛋白结合率的测定
Determination of plasma protein binding rate of multicomponent in scutellaria baicalensis Georgi
摘要
Abstract
Aim To establish a RP-HPLC method for determination of concentration of four major flavonoids, namely baicalin ( BL ), wogonoside ( WL ), wogonin ( W ) and oroxylin A ( OA ), in the total flavonoids extract of scutellaria baicalensis Georgi in rat plasma and to study the plasma protein binding rate. Methods The equilibrium dialysis was carried out to determine the plasma protein binding rate of the target compounds. The plasma samples were extracted by protein precipitation with methanol. With the use of maackiain as the internal standard, the concentration of the multicomponent inside and outside the dialysismembranes was determined by HPLC method. Results The plasma protein binding rates of baicalin and wogonoside decreased with increasing of concentration in a concentration-dependent manner( BL: 82.03% →77.25% →67. 17% ; WL: 82. 24%→ 76. 08% →69. 87% ), while were not the same of wogonin and oroxylin A. The average plasma protein bingding rates of wogonin and oroxylin A were ( 89. 66 ± 1. 19 )% and ( 88. 56 ± 1. 87 )% , respectively. Conclusions The HPLC method established in the paper is simple, sensitive, little interfe-rential and stable. The four major compounds in the total fla-vonoids extract of scutellaria baicalensis Georgi have high capacity in binding plasma protein in rat in vitro.关键词
黄芩/黄芩苷/汉黄芩苷/汉黄芩素/千层纸素A/血浆蛋白结合率/平衡透析法Key words
scutellaria baicalensis Georgi/ baicalin/ wogonoside/ wogonin/ oroxylin A/ the plasma protein binding rate/the equilibrium dialysis分类
医药卫生引用本文复制引用
冯志强,韩坚,谢智勇,廖琼峰,张蕾..黄芩提取物中黄酮类成分血浆蛋白结合率的测定[J].中国药理学通报,2012,28(2):286-289,4.基金项目
国家自然科学基金项目(No 30901953) （No 30901953）
国家教育部新教师基金项目(No 20094425120001) （No 20094425120001）
"重大新药创制"国家科技重大专项(No 2009ZX09304-003,2009ZX09501-017) （No 2009ZX09304-003,2009ZX09501-017）
