中国药理学通报2012，Vol.28Issue(2)：289-293,5.DOI:10.3969/j.issn.1001-1978.2012.02.032
昆明小鼠焦虑与抑郁动物模型相关性研究:明暗箱实验与悬尾实验
Correlation between anxiety and depression in animal models: evidence from light/dark box and tail suspension test in Kunming mice
摘要
Abstract
Aim To explore the correlation of light/dark box ( LDB ) and tail suspension test( TST), as animal models of anxiety and depression in Kunming mice. Methods The behavior of adult, male, Kunming mice in LDB and TST was recorded by sequence, for five or six minutes, with a one-week inter-trial interval. The following parameters were evaluated: percentage of time exploring in the LDB light area( LDB_Ltime% ); percentage of squares crossing in the LDB light area( LDB_Lcross% ); and percentage of rears in the LDB light area( LDB_Lrear% ). Subsequently, factor analysis, cluster analysis, correlation analysis , consistency check and survival analysis were calculated for these parameters. Results ① Factor analysis and cluster analysis revealed that variances of LDB had contribution to anxiety, and variances of TST had loading on depression. ② Correlation analysis displayed a good intercorrelation between variances of either LDB or TST, but a poor intracorrelation between these of LDB and TST. Both ICC and kappa reflected a poor consistency on evaluation of LDB and TST. ③ No significant difference be-tween LDB-and TST-median survival of the latence parameter was found. Conclusions LDB parameters load mainly on anxiety factor, and TST parameters have large loading on depression factor; however, there are poor correlation and consistency on LDB and TST. To answer the question, "correlation of animal models between trait anxiety and state anxiety" and " correlation of animal models between anxiety and depression , novel animal models and synthetic evaluation methods should be founded, and new strains should be selected. Furthermore, emphasis should be placed on combination as a "behavionomics" and on "pattern recognition of constructs of multiple animal models when drugs were evaluated.关键词
昆明小鼠/焦虑/抑郁/动物模型/明暗箱实验/悬尾实验/相关性/行为组学/模式识别/因子分析/聚类分析/相关分析/组内相关系数/Kappa一致性系数/生存分析Key words
Kunming mice/ anxiety/ depression/ animal model/ light/dark box/ factor analysis/ cluster analysis/correlation analysis/ intraclass correlation coefficient/ kappa agreement coefficient/ survival analysis分类
医药卫生引用本文复制引用
孙世光,李子峰,刘健,张惠云,乔明琦..昆明小鼠焦虑与抑郁动物模型相关性研究:明暗箱实验与悬尾实验[J].中国药理学通报,2012,28(2):289-293,5.基金项目
国家自然科学基金资助项目(No 30930110,30973688) （No 30930110,30973688）
国家重点基础研究发展计划(973计划)资助项目(No 2011CB505102) （973计划）
山东省中医药科技发展计划项目(No 2011-107) （No 2011-107）
