实用医学杂志2011，Vol.27Issue(23)：4194-4196,3.DOI:10.3969/j.issn.1006-5725.2011.23.006
褪黑素对小鼠缺血再灌注脑损伤后海马CA3区神经元的保护作用
Protection of melatonin to CA3 hippocampus neurons of mice on cerebral ischemia-reperfusion injury
摘要
Abstract
Objective To observe the effect on learning and memory abilities of mice on ischemia -reperfusion brain injury, and discuss the protection of Melatonin (MT) on hippocampus neurons of mice after cerebral ischemia-reperfusion injury. Methods 60 healthy mouse were randomly assigned to control group , model group and model treatment group. Model group were adopted by the method of bilateral carotid artery occlusion, the treatment group were injected MT (20 mg-kg-1 three times daily) by intraperitoneal injection,the control group was given equivalence physiological saline simultaneously. Each group were detected the abilities of learning and memory by Y-type maze three days before sacrificed. Then SOD , NO, MDA and the water content of brain tissues were detected and the change of hippocampus CA3 neurons ultra-structural was observed by electron microscopy.Results Compared with the control group, the ability in learning and memory of treatment group was obviously improved (P < 0.05). Biochemical detection, the content of SOD in treatment group was obviously increased (P < 0.05),while the contents of NO、MDA were sensibly decreased(P< 0.05). By electron microscope, observation the injury of hippocampus neurons in treatment group has been greatly in proved than the non-treated groups. Conclusions MT could significantly improve the mice abilities of learning and memory on ischemia-reperfusion brain injury. And MT might have the neuroprotection effect.关键词
缺血再灌注/褪黑激素/学习记忆/海马/超微结构/小鼠Key words
Ischemia-reperfusion/Melatonin/Learning and memory/Hippocampus/Ultra-structure/Mice引用本文复制引用
肖朝伦,余资江,李玉美,令狐艳,余彦,康朝胜,常傲霜..褪黑素对小鼠缺血再灌注脑损伤后海马CA3区神经元的保护作用[J].实用医学杂志,2011,27(23):4194-4196,3.基金项目
贵州省教育厅自然科学研究项目( 编号:黔教科2009-0139 号) （ 编号:黔教科2009-0139 号）
贵阳市科技局计划项目[编号:筑科计合同字第2008-15-21 号 （）
(2010)黔科农合同字第1-社-11 号] （2010）
贵阳医学院院基金(编号:院基金合同字2009-30 号) （编号:院基金合同字2009-30 号）
