中国实验动物学报2011，Vol.19Issue(5)：410-414,5.DOI:10.3969/j.issn.1005-4847.2011.05.011
树鼩Retn基因的克隆及序列测定
Cloning and sequencing of Retn gene in tree shrew
摘要
Abstract
Objective The aim of this study was to clone the Retn gene of tree shrews to provide basic data for further research. Methods Total RNA was extracted from adipose tissue of tree shrews. Retn gene was amplified by RT-PCR and cloned. The sequence of Retn gene was identified by sequencing and enzyme digestion. Results The total RNA had good integrity. The expected 363 bp fragment was obtained by RT-PCR. The Retn gene was cloned in vector and identified by enzyme digestion (Pst I). 363 nucleotide acids encoded an open reading frame with 114 amino acids. The sequence has been submitted to GenBank (No. JF267792). The results showed a 95% similarity in nucleotide acids and 99% in amino acids with mouse Retn gene. Conclusion We have first reported the Retn gene of the tree shrew, and lay a foundation for further study on biological functions of Retn gene in tree shrews.关键词
树鼩/Restn基因/克隆/测序Key words
Tree shrew/ Retn gene/ Clone/ Sequence分类
生物科学引用本文复制引用
鲁帅尧,和占龙,禹文海,赵远,陈瑜,李鸿钧..树鼩Retn基因的克隆及序列测定[J].中国实验动物学报,2011,19(5):410-414,5.基金项目
云南省应用基础研究面上项目(编号:2009ZC185M). （编号:2009ZC185M）
