中国水产科学2011，Vol.18Issue(6)：1219-1225,7.DOI:10.3724/SP.J.1118.2011.01219
IGF-Ⅰ及其受体基因在牙鲆胚胎发育过程中的表达图式
Expression patterns of IGF-Ⅰ and its receptor genes during embryonic development of Japanese flounder
摘要
Abstract
Insulin-like growth factor I (IGF-I) is an important regulator of growth and development in vertebrates, and its biological actions are mediated by binding to membrane-specific IGF-I receptors (IGF-Irs). Therefore, the analysis of IGF-I and IGF-IR expression can help to further elucidate the role for IGF in the regulation of developmental processes. In this study, the expression patterns of IGF-I and its receptor genes during the embryonic development of Japanese flounder (Paralichthys olivaceus) were analyzed by fluorescent real-time PCR using embryos at different developmental stages. We observed that maternal transcripts of IGF-I, IGF-IR-1 and IGF-IR-2 were present in early embryos and that their expression is developmentally regulated at different embryonic stages. A few IGF-I mRNAs are detected in early embryos, and the zygotic IGF-I transcripts are gradually increased from the eye lens formation stage to the pre-hatching stage. IGF-IR-1 and IGF-IR-2 exhibit distinct temporal expression patterns during embryonic development. IGF-IR-1 has fewer transcripts than IGF-IR-2, and these appear at the fertilized egg, cleavage and blastula stages. The zygotic IGF-IR-1 transcripts are abundantly expressed at the gastrula stages and maintain a high level at the neurula, eye lens formation, heart beating and pre-hatching stages. By contrast, the considerable transcripts of IGF-IR-2 are detected as early as the fertilized egg through to the gastrula stage, but the zygotic IGF-IR-2 transcripts decrease significantly following embryogenesis. This indicates that the two IGF-I receptors might play different functional roles during embryonic development of Japanese flounder.关键词
牙鲆/IGF-Ⅰ/IGF-Ⅰ受体/胚胎发育/表达图式Key words
Paralichthys olivaceus/ IGF-I/ IGF-I receptors/ embryonic development/ expression patterns分类
农业科技引用本文复制引用
张俊玲,施志仪,程琦,翟万营..IGF-Ⅰ及其受体基因在牙鲆胚胎发育过程中的表达图式[J].中国水产科学,2011,18(6):1219-1225,7.基金项目
国家自然科学基金项目(31172392) （31172392）
上海市重点学科水生生物学建设项目(S30701) （S30701）
上海海洋大学博士启动基金资助项目. （）
