安徽农业科学2011，Vol.39Issue(28)：17152,17160,2.
玉米抗黄曲霉Ti基因的克隆与表达
Clone of the Maize Gene Ti with the Resistance to Aspergillus flavus and its Expression
摘要
Abstract
[Objective] The clone of maize gene Ti ( Trypsin inhibitor gene Ti)and its expression after introduction was conducted, which would lay the basis of its research in future. [ Method ] The concentration and purity of total RNA, extracted from the seed of maize variety Kanghuang No. 3, were detected through the technique of electrophoresis. The maize gene Ti was amplified by means of RT-PCR system and the target genp was collected. The target gene connected with the vector PMD18-T was transferred into the E.coli competent cells, and then, (be recombined plasmid DNA was extracted and identified after its enzymatic digestion. The recover; gene Ti connected with the expression vector PCEX-4T-1 was introduced for its expression and the expression products was measured. [Results] The trypsin inhibitor appeals wilh the form of its dimer,which had also inhibition in vitro to the germination of spore- and hyphae growth of Aspergilhis flavus. [Concluuon] The important role of the trypsin inhibitor in the resistance-improving of corn to Aspergillus flavus was understood through the research on com gene Ti.关键词
玉米/抗黄典霉基因/克隆与表达Key words
Corn/ Aspergillus flavus/ Clone and expression分类
农业科技引用本文复制引用
李依韦,薛兰兰..玉米抗黄曲霉Ti基因的克隆与表达[J].安徽农业科学,2011,39(28):17152,17160,2.基金项目
内蒙古民族大学(BS018). （BS018）
