高校化学工程学报2011，Vol.25Issue(5)：897-903,7.
水蒸汽蒸馏法提取湿地松松针中挥发油和莽草酸的研究
Study on Extraction of the Volatile Oil and Shikimie Acid from Pinus Elliottii Engelm Needles by Steam Distillation
摘要
Abstract
The volatile oil and shikimic acid can be extracted from pinus elliottii engelm needles by means of steam distillation, and the content of shikimic acid can be determined by high performance liquid chromatography (HPLC). By the single-factor experiments and orthogonal test, the optimum extraction conditions were confirmed as follows: the granularity of pinus elliottii engelm is 40 mesh, solid-liquid ratio (W/V) is 1 : 12, the soaking time is 6 h and the extracting time is 4 h; under above extracting conditions, the extraction ratio of the shikimic acid is 1.32%. 133 chemical components were identified from the volatile oil extracted from the pinus elliottii engelm needles when it was analyzed by gas chromatography-mass spectrometry (GC_MS); of which 37 elements covering 88.33% of the total volatile oil ion current were identified. The principle components of the volatile oil are as follows: β-Pinene (15.08%), Germacrene (14.31%), α-Pinene (8.67%), βCaryophyllene (9.82%), 3-Carene (5.49%), τ-Muurolene (5.33%), Cadinene (4.98%), α-cadinol (3.77%),γ-Elemene (3.47%) and α-Caryophyllene (2.13%). The total oil productivity of the volatile oil of pinus elliottii engelm needles is 0.315%. This study could provide the base for deeper development of the pine needle medicinal resources.关键词
湿地松针/莽草酸/高效液相色谱/水蒸馏浸提法/芷交试验/气相色谱-质谱Key words
pinus elliottii engelm/ shikimic acid/ high performance liquid chromatography, steam distillation/ orthogonal test/ gas chromatography-mass spectrum分类
化学化工引用本文复制引用
谢济运,陈小鹏,李志荣,陈芳,粟本超..水蒸汽蒸馏法提取湿地松松针中挥发油和莽草酸的研究[J].高校化学工程学报,2011,25(5):897-903,7.基金项目
国家自然科学资助项目(20976031,31060102) （20976031,31060102）
广西自然科学基金资助项目(0991030) （0991030）
广西研究和教育创新计划项目(2008105930817M46) （2008105930817M46）
广西教育厅科学研究项目([2006]26). （[2006]26）
