安徽农业科学2011，Vol.39Issue(23)：14059-14061,14064,4.
研究土壤微生物多样性的PCR条件优化
Optimization of PCR Condition in Soil Microbial Diversity Study
摘要
Abstract
[Objective] The research aimed to optimize the condition of PCR to analyze genetic diversity of soil microorganism. [Method]Different PCR systems were set up by altering the factors, such as primer design, volume, annealing temperature and cycles. [Result] In the optimized PCR system, specific, sensitive and stable amplified bands were obtained when cycles were 35, the annealing temperature was 55 ℃ ,the volume of PCR was 20 μl. [Conclusion] The optimized PCR reaction system had high specificity and reliability.关键词
PCR-DGGE/土壤微生物/多样性Key words
PCR-DGGE/Soil microorganisms/ Diversity分类
农业科技引用本文复制引用
邸宁,刘志民,马焕普..研究土壤微生物多样性的PCR条件优化[J].安徽农业科学,2011,39(23):14059-14061,14064,4.基金项目
北京市教委资助项目(KM200910020001). （KM200910020001）
