安徽农业科学2011，Vol.39Issue(23)：13949-13951,3.
适用于川芎的ISSR-PCR反应体系的建立及优化
Establishment and Optimization of ISSR-PCR Amplification System for Ligusticum chuanxiong Hort
摘要
Abstract
[Objective] This study was to establish and optimize the ISSR-PCR reaction system for Ligusticum chuanxiong Hort. [ Method] Using genomic DNA of Chuanxiong leaf extracted via an improved CTAB method, single factor analysis was performed to investigate the impacts of DNA template concentration, Mg2+ concentration, dNTPs concentration, primer concentration, Taq DNA polymerase concentration on ISSR-PCR amplification and to optimize this system for Ligusticum chuanxiong Hort. [ Result] The ISSR-PCR amplification(25 μl) suitable for Ligusticum chuanxiong Hort. Was determined to be composed of 2. 5 μl of 10 × reaction buffer, 2.1 mmol/L MgCl2, 300 μmol/L dNTPs, 0.4 μmol/L primer, 1.0 U Taq DNA polymerase and 20 -40 ng genomic DNA. [ Conclusion] Our study laid basis for analyzing the genetic diversity of Ligusticum chuanxiong Hort. Resources distributed in 17 different areas of China.关键词
川芎/ISSR-PCR/体系优化Key words
Ligusticum chuanxiong Hort. /ISSR-PCR/Parameter optimization分类
农业科技引用本文复制引用
王岚,唐琳..适用于川芎的ISSR-PCR反应体系的建立及优化[J].安徽农业科学,2011,39(23):13949-13951,3.基金项目
“十五”国家科技攻关项目(2004BA721A31). （2004BA721A31）
