法医学杂志2011，Vol.27Issue(5)：321-323,3.DOI:10.3969/j.issn.1004-5619.2011.05.001
家兔死后心肌酰胺A带的傅里叶变换红外光谱图像分析
Analysis of the Changes of Amide A in Rabbit Heart after Death by FTIR Spectroscopy
摘要
Abstract
Objective To explore the relationship of changes of amide A in rabbit heart and the postmortem interval (PMI) by FTIR spectroscopy technique. Methods Thirty-two rabbits were sacrificed and the hearts were sampled at 20℃ within 48 h postmortem points. All samples were sliced and tested by FTIR spectroscopy technique. The images of amide A were created by FTIR spectroscopic imaging. The positive and negative area ratios of amide A were analyzed using imaging analysis system. Results The positive and negative area ratios declined regularly with the prolongation of death time in 48 h. There was a significant quadric relationship between the area ratios(y) of amide A(positive and negative area) and PMI(x). The regression equation was y=0.001 x2-0.038x+0.747(R2=0.940). Conclusion The ratios of positive and negative area of amide A showed a strong correlation with PMI and could be used to estimate PMI.关键词
法医病理学/谱学,傅里叶变换红外/心肌/酰胺A带/死亡时间/兔Key words
forensic pathology/ spectroscopy, Fourier transform infrared/ myocardium/ amide A/ postmortem interval/ rabbits分类
社会科学引用本文复制引用
张海东,杨天潼,郑娜,孙婷怡,刘良..家兔死后心肌酰胺A带的傅里叶变换红外光谱图像分析[J].法医学杂志,2011,27(5):321-323,3.基金项目
国家自然科学基金青年科学基金项目(81001349) （81001349）
