林产化学与工业2011，Vol.31Issue(5)：60-64,5.
茄尼醇与牛血清白蛋白相互作用的研究
Interaction between Solanesol and Bovine Serum Albumin
摘要
Abstract
Under simulated physiological conditions, the. Interaction between solanesol and bovine serum albumin (BSA) was investigated. The quenching constant, binding constant, the number of binding site and binding distance were determined by fluorescence spectrnscopy and ultraviolet absorption speclroscopy. They were 1.76×107 and 2.7×107 mol/L, 1. 54 x 106 and 1.9 X 106 mol/L, 1.09 and 1.50, and r =0nm at 25℃ and 37℃, respectively. Thermodynamic parameters were △G < 0,△H >0, AS >0. So the fluorescence of bovine serum albumin was strongly quenched by solanesol in the static quenching style. Thermodynamics analysis demonstrated that the hydrophobic interaction force played a main role in the binding of solanesol with bovine serum albumin and solanesol was inserted into bovine serum albumin at tryptophan 212.关键词
荧光光谱法/紫外吸收法/茄尼醇/牛血清白蛋白/荧光猝灭Key words
fluorescence spectroscopy/UV spet'trnscopy/solanesol/bovine serum albumin/fluorescence quenching分类
化学化工引用本文复制引用
高义霞,周向军,王风霞,张花..茄尼醇与牛血清白蛋白相互作用的研究[J].林产化学与工业,2011,31(5):60-64,5.基金项目
陇南地区灾后住宅重建与特色农产品快速生产技术集成及示范项目(2008BAK51B05) （2008BAK51B05）
国家自然科学基金资助项目(50773064) （50773064）
