法医学杂志Issue(6)：405-408,412,5.DOI:10.3969/j.issn.1004-5619.2011.06.002
氯胺酮对PC12细胞增殖及凋亡的影响
Effects of Ketamine on Proliferation and Apoptosis of Pheochromocytoma Cell
左媛宜 1赵彦伯 2蒋小岗 3顾振纶 4郭次仪 3卞士中1
作者信息
- 1. 苏州大学医学部法医学系,江苏苏州215123
- 2. 苏州大学司法鉴定所,江苏苏州215000
- 3. 苏州中药研究所,江苏苏州215006
- 4. 昆山市公安局 刑警大队法医室,江苏昆山215300
- 折叠
摘要
Abstract
Objective To explore the effect of ketamine on adrenal pheochromocytoma(PC12) cell proliferation inhibition and induction of apoptosis and its mechanism. Methods PC 12 cells of rats were models for dopaminergic neuron. PC12 cells were cultured with ketamine at concentrations of 0.9, 1.2, 1.5, 1.8 and 2.1 mmol/L, respectively. The cell viability was measured by MTT method after incubation at 12, 24, 48 and 72 h. Hoechst stain was used to observe the morphological changes of apoptosis. PC 12 cells cultured after 48 h with different concentrations of ketamine were selected to detect apoptotic rate using flow cy-tometry and detect the expression of bax and bcl-2 proteins using Western blotting. Results For different concentrations of ketamine, vitality of PC 12 cells significantly decreased with increase of the incubation time. Apoptosis was obviously observed using Hoechst staining. Flow cytometry showed that apoptosis rates significantly increased with increasing ketamine concentrations. Conclusion Ketamine can inhibit the proliferation of PC12 cell by inducing apoptosis of the PC 12 cell in a concentrations-dependent manner. The underlying mechanism may be related to promoting the expression of bax and inhibiting the expression of bcl-2 in the cells.关键词
法医病理学/氯胺酮/PC12细胞/细胞增殖/细胞凋亡/大鼠Key words
forensic pathology/ ketamine/ PC12 cells/ cell proliferation/ apoptosis/ rats分类
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左媛宜,赵彦伯,蒋小岗,顾振纶,郭次仪,卞士中..氯胺酮对PC12细胞增殖及凋亡的影响[J].法医学杂志,2011,(6):405-408,412,5.
