安徽农业科学2011，Vol.39Issue(22)：13378-13379,2.
高效液相色谱法测定消浊通淋颗粒中槲皮素和山奈素的含量
Determination of Quercetin and Kaempferol Contents in Xiaozhuotonglin Granules by HPLC
李洪 1张静 1高玉梅 2王晓欣 1张胜国3
作者信息
- 1. 河北化工医药职业技术学院,河北石家庄050026
- 2. 河北工程大学,河北邯郸056002
- 3. 河北省食品药品监督管理局教育培训中心,河北石家庄050030
- 折叠
摘要
Abstract
[Objective] The research aimed to establish a method for the determination of quercetin and kaempferol contents in Xiaozhuotonglin granules. [Method] The contents of quercetin and kaempferol in Xiaozhuotonglin granules was determined by using HPLC. The separation was performed on SHIMADZU VP-ODS c (250.0 mm ×4.6 mm,5μn). The mobile phase was methanol -0.4% phosphoric acid solution (48:52) at the flow rate of 1.1 ml/min. The defection wavelength was 360 nm. [ Result ] The linear range of quercetin was in the range of 0.075 2 -0.2705 μg, the addition recovery rate was 99.5% ,RSD =0.30% (n=6), the linear range of kaempferol was in the range of 0.113 0 - 0.406 8 μg, the addition recovery rate was 99.7% ,RSD=0. 34% (n=6). [Conclusion] The method for the determination of quercetin and kaempferol in Xiaozhuotonglin granules by HPLC is simple, rapid, good repeatability and accurate, and it is suitable for the determination of Xiaozhuotonglin granules.关键词
消浊通淋颗粒/高效液相色谱/山奈素/槲皮素/含量测定Key words
Xiaozhuotoonglin granules/ HPLC/ Kaempferol/ Quercetin/Content determination分类
农业科技引用本文复制引用
李洪,张静,高玉梅,王晓欣,张胜国..高效液相色谱法测定消浊通淋颗粒中槲皮素和山奈素的含量[J].安徽农业科学,2011,39(22):13378-13379,2.
