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6+1STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验

平原 周怀谷 许炎 夏子芳 郑卫国

法医学杂志Issue(6):444-446,3.
法医学杂志Issue(6):444-446,3.DOI:10.3969/j.issn.1004-5619.2011.06.012

6+1STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验

Rapid DNA Identification Using 6+1 STR Kit and EX-Q20 Electrophoresis

平原 1周怀谷 2许炎 1夏子芳 1郑卫国3

作者信息

  • 1. 上海市公安局物证鉴定中心 上海市现场物证重点实验室,上海200083
  • 2. 复旦大学 生命科学学院,上海 200433
  • 3. 无锡中德美联生物技术有限公司,江苏无锡214174
  • 折叠

摘要

Abstract

Objective To establish a rapid STR genotyping method for individual identification. Methods Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and SinofilerTM Kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared. Results 6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time. Conclusion 6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.

关键词

法医遗传学/短串联重复序列/个体识别/快速检验

Key words

forensic genetics/ STR/ individual identification/ rapid identification

分类

社会科学

引用本文复制引用

平原,周怀谷,许炎,夏子芳,郑卫国..6+1STR试剂盒和电泳凝胶EX-Q20用于DNA快速检验[J].法医学杂志,2011,(6):444-446,3.

法医学杂志

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1004-5619

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